Agronomic efficiency is one of the recent strategies to fight zinc (Zn) deficiency in which agronomic managements such as application of organic and chemical fertilizers are used to enhance availability of Zn for plants. Chemical and organic fertilizers affect soil chemical and physical properties, particularly chemical forms of zinc in soil solid and solution phases and thereby improve soil Zn availability. This field study aimed to evaluate the effects of organic fertilizers, industrial waste and zinc sulfate on different zinc fractions in solid and solution phases in two successive years in Rudasht Research Field, Isfahan. In addition, correlation between the fractions of Zn in soil and grain zinc concentration was established. Treatments consisted rubber powder (1 t ha -1 ), rubber ash (200 kg ha -1 ), sewage sludge (5 and 10 t ha -1 ), cow manure (5 and 10 t ha -1 ), ZnSO 4 (40 Kg ha -1 ). A without added Zn treatment was also considered as control. Three weeks after applying Zn fertilizer treatments, Back Cross (zinc-efficient) and Kavir (zinc-inefficient) wheat genotypes were seeded in each plot. After harvesting wheat plants, at full maturing of the seed stage, grain zinc concentration was measured. Soil samples were taken from the rhizosphere to evaluate the effects of treatments on chemical Zn forms in solid and solution phases of rhizosphere soil. After measuring cations, anions and dissolved organic carbon (DOC) concentration, Zn species in soil solution was estimated using Visual MINTEQ model. The chemical forms of Zn in soil solid were extracted according to the Tessier' sequential extraction procedure. The results showed that zinc species in solid and solution phases were affected by Zn fertilizer treatments, year and cultivars. Dominant species of Zn in soil solution were Zn 2+ , Zn-DOC, ZnCO 3 °, ZnCl + , and ZnOH + , respectively. Among Zn species in solution, free cation Zn 2+ was more affected by 10 t ha -1 cow manure treatment. Distribution of Zn-DOC in rhizosphere soil of 'Back Cross' was greater than 'Kavir'. Concentration of DOC was also affected by Zn fertilizer treatments and wheat genotypes and it was higher in rhizosphere soil of 'Back Cross' than 'Kavir'. Sewage sludge at 10 t ha -1 had the highest effect on DOC concentration. Concentration of DOC was correlated significantly with grain zinc concentration. There was a strong correlation between Zn 2+ , ZnCO 3 °, and particularly Zn-DOC and grain zinc concentration. This result shows that Zn-DOC has a decisive role in Zn availability for plant. Distribution of Zn in solid phase decreased in the following order: residual carbonate Fe, Mn-oxide organic bound exchangeable. Sewage sludge at the 10 t ha -1 applied rate has the most effect on all fractions of soil Zn in wheat rhizosphere. Also, all chemical forms of Zn except Zn bound to carbonate at the first year and residual fraction at the second year was correlated with grain Zn concentration.