The temperature response of N mineralization in aggregate scale was investigated. The study was conducted in soils collected from natural oak and deforested counterparts of a forest ecosystem in the west-central Iran. In the first study, the effects of land use change on the concentration of ammonia, nitrate, total inorganic N were measured. Urease activity was determined and biological index of N availability were measured. In the second experiment, the soils the forest and deforested counterparts were subjected to wet sieving procedure. Three distinctive aggregate sizes including 0.05-0.25, 0.25-1 and 1-2 mm were separated. The concentration of ammonia, nitrate, total inorganic N, urease activity and biological index of N availability were measured in the separated aggregates. In the third experiment, the temperature responses of ammonification, nitrification, N mineralization rates, biological index of N availability and urease activity were measured. For this purpose, an incubation was run and the above mentioned parameters were measured at 7, 25 and 35 °C under optimum moisture condition, following 60 days of incubation. Results indicated that deforestation caused a significant decrease in the concentrations of ammonia, nitrate, total inorganic N, biological index of N availability and urease activity. However, the decrease was happened in a less extent in the case of nitrate concentration. It was also demonstrated that regardless of ammonia concentration , other factors are significantly influenced by aggregate size distribution so that the values were consistently greater in 1-2 mm aggregates. Land use and temperature significantly influenced the rates of N fluxes as well as urease activity. Expectedly, increasing incubation temperature resulted in enhancement of the N flux rates however, the response rates were depended on the aggregate size. It was observed that an increase in incubation temperature increased the rates of fluxes in 1-2 mm aggregates while a decrease was monitored in the 0.05-0.25 mm size Keywords: N mineralization, ammonification, nitrification, biological index of N availability, temperature response, temperature-quality hypothesis