Since particles of atmospheric deposition are fine, they have a high adsorption capacity and can, therefore, easily translocate the contamination to other areas. Plants are able to absorb certain pollutants through their leaves and accumulate them in their biomass. Therefore, biological tracers using plant tissues could replace the physical methods and be used to assess the level of air pollution. Isfahan is one of the most industrialized cities in Iran where air pollution is a major issue. The objectives of this study were to evaluate the feasibility of using pine needles as bioinicators for air pollution and also to investigate the spatial and temporal variability of atmospheric pollution in Isfahan city and to determine the level of pine needles contamination with heavy metals based on their magnetic susceptibility. Pine needles samples were taken monthly for a period of 8 months from 30 sites across the city of Isfahan. There were 4 treatments including unwashed + unwashed (UU), unwashed + washed (UW), washed + unwashed (WU) and washed + washed. The total concentration of Fe, Zn, Cu, Co and Ni was measured after dry digestion and that of Pb after wet digestion. An atomic absorption spectrometer was employed to determine the concentration of heavy metals. Besides, the magnetic susceptibility of all the leaf samples was determined using a MS2B sensor. The results indicated that the concentration of heavy metals in pine needles followed the order Fe Zn gt;Cu Ni Co and fall ring summer. Besides, the concentration of heavy metals in pine needles with different treatments followed the order UU WU UW WW. To determine the relationship between the concentration of heavy metals in pine needles and that in atmospheric dust, the data reported by Norouzi (2016) was used. The results showed that there was a significantly high correlation between the concentration of heavy metals in pine needles and that in atmospheric dust reported by Norouzi (2016). Therefore, if no information is available, pine needles could be well used to evaluate the pollution level of atmospheric deposition. Box plots of the concentration of heavy metals in different months throughout the sampling period indicated that the level of heavy metals increased from April to November and sharply declined due to a heavy rainfall occurred. The highest correlation was obtained between the concentration of Fe and that of Cu, Zn, Co, Ni, and Pb indicating their common source. The spatial variability of heavy metals shows that the city center and other areas with heavy traffic have higher concentration as compared to low traffic areas and suburbs. Based on the high correlation between the concentration of heavy metals and the magnetic susceptibility of pine needles, it appears that a fraction of atmospheric dust is the result of the linkage between the heavy metals into the lattice structure of ferrimagnetic particles during the formation process or afterward through the other anthropogenic parameters. In conclusion, pine needles could be used as an efficient bioindicator for the evaluation of atmospheric pollution with heavy metals. Keywords : Bioindicators, pollution, heavy metals, magnetic susceptibility, Isfahan city