According to statistical forecasts, the world's population is expected to increase to 9 billion by 2050 that will increase the demand for protein products, especially the limitation of digestible protein resources is an important issue. The hypothesis of this research are: 1) quantity and quality of Azolla extract could be affected by the extraction procedures, 2) the application of Azolla extract will improve the growing conditions of cucumbers as an organic fertilizer and plant biostimulator, and 3) degradation kinetic parameters of different extraction of Azolla and powder of dried Azolla will be distinct. In this study, Caroliniana Azollaceae was used to optimize protein extraction protocol and the other experimental procedures. Analysis of variance, comparison of the protocols and application of the extract in Cucumber growing media were done by completely randomized design with a one-way treatment structure using SAS 9.0 software. The kinetic models werefit by GraphPad Prism 6.02 software. The results of first study showed that using of high speed homogenizer, acidic pH with a tannin bonding adsorbent and heat and pressure treatments increased the quantity of the extracted protein and changed the pattern of extratedamino acids. The results of the second study showed that the application of Azolla extract in the cucumber culture medium in Johnson solution with 50% lower concentrations of nitrogen and iron, conserved the yield, fresh weight and dry weight of the plant compared to the control (complete Johnson solution). But reproductive growth and flowering time of plants and fruit ripening have been decreased and the concentration of iron in fruits and roots and zinc in roots increased. The results of the third study showed that the addition of 2% carbon in the form of dried Azolla powder to the soils induced carbon mineralization potential (Y max ) and reduced the decomposition rate constant (K 1 ) compared to the 1% carbon treatment. Addition of Azolla extraction contained 0.6% carbon to the soils increased the carbon mineralization potential and the decomposition rate constant compared to the extract contained 0.3% carbon. Additionally, comparison of the kinetic parameters of dried powder decomposition and the extractionshowed that the carbon mineralization potentials (Y max ) and the decomposition rate constants (K 1 ) of the dried powder were higher than of the extract Keywords : Caroliniana Azollaceae , Extraction protocols, Protein, Amino acids, Organic fertilizer and plant biostimulator, Green house Cucamber, Degradation kinetic parameters