The edible mushroom, known as Agaricus bisporus , is the most important and commonly used specie in the world, cultivated commercially and has the largest share in the mushroom production in our country as well. Mushrooms are rich sources of protein, fiber, vitamins and minerals, while they can grow on agricultural waste composts. On the other hand, thousands of tons of agricultural wastes are burnt or discarded every year in Iran, causing pollution in the environment. One of the proper ways to recycle these waste materials is to use them as feed stalks for mushroom compost. Therefore, to investigate the possibility of mushroom compost production from some agricultural waste, this research was conducted in agriculture research fields of Isfahan University of Technology. At the first stage of the experiment some agricultural wastes were collected and characterized. The wastes were; pruned branches of grape and almond trees, wheat straw and sugar cane bagasse. To enrich the wastes, some chicken manure was also used. The wastes and the manure samples were analyzed for some physical and chemical properties. In terms of nutrient elements, the manure was significantly richer than the pruning wastes. In the next stage the plant residues as different treatments mixed with about 10% by weight of chicken manure as starter and incubated at about water holding capacity moisture content and room temperature for composting. During the composting process the residues were aerated by hand mixing and the moisture was provided by water spray, if necessary, every two weeks. The composting materials were; grape pruning chips, almond pruning chips, grape-almond mixture, grape-almond-bagasse mixture and grape-almond- wheat straw mixture. The maturity of the composts was examined using seed germination test. The composts were then analyzed for different physico-chemical properties. A mushroom compost sample from Sepahan's Yekta mushroom farm was also analyzed as a reference for comparison. The results showed that in terms of nutrient elements, the produced composts were all significantly poorer than the reference sample, so in the third step of the experiment, the produced composts were enriched with some 25% chicken manure, 1% urea and 5% gypsum and incubated for two more weeks. The enrichment improved the pH, EC and nutrient contents of the compost samples to about the reference levels. The produced composts then posturized and incubated with the Agaricus bisporus spawns and placed in optimum conditions of moisture and temperature of a commercial mushroom farm. The mushroom mycelium could not grow on the produced composts that could be due to different factors. Too much maturity and biologically inactive condition of the composts may be the main reason for this failure. Keywords: Mushroom substrate, Agricultural waste, Nutrient enrichment, Compost maturity, Chemical properties.