The hulled wheats are among the earliest domesticated Triticeae by humankind. Hulled wheats have thickened tough glumes and during threshing the spike will break up in to spikelets in which the grain is surrounded by the tough glumes. In the free-threshing wheats the glumes are thin and easily broken off, and the chaff will separate from the grain easily. A single gene, the Q factor, localized on long arm of chromosome 5A, is responsible for hulledness in many genotypes of wheats. This type of wheat can be found with different ploidy levels including the diploid einkorn (2n=2x), tetraploid emmer (2n=4x), and hexaploid spelt (2n=6x).They possess many agronomic traits such as abiotic stress tolerances (salt, drought and heat), biotic stress tolerances (powdery mildew, rusts and fusarium head blight), higher grain protein quality and quantity, and higher micronutrient concentrations (Zn, Fe and Mn). These characteristics has made hulled wheat a useful germplasm resource and highly valuable in wheat breeding programs. Hulled wheats are found in different regions of Iran but their genetic potential is not exploited owing to lack of genomic information. Two hulled wheat accessions (Zarne and Jonghan) collected from farmers filed in two distantly located villages in the Chehar-Mahal Bakhtiari province seemed to hold characteristics valuable for wheat improvement. Preliminary cytogenetic studies revealed the tetraploid nature of both accessions and dismissed our initial presumption that they are hexaploid spelt. To elucidate the genetic relationships with other wheats, we compared the two hulled accession with other 28 diploid, tetraploid and hexaploid wheat genotypes using seventeen Simple Sequence Repeats (SSR) primers. The SSR markers, designated as XGWM, were obtained from a previous report mapping them on the A and B genomes of wheat. These primers generated between 2 to 11 allels per locus and the average number of alleles per locus was 4.36. The value for allelic polymorphism information content (PIC) ranged from 0.25 to 0.85 with an average of 0.53 per locus. The high value of PIC showed that a high level of polymorphism was present for accessions dir=ltr Key words: hulled wheat, PIC, SSR primer, PCA
