Salinity is one of the most important limiting factors for safflower production in Isfahan. Inoculation of safflower with microorganism such as mycorrhizal fungi may increase salt tolerance in this species. Therefore, the purpose of this study was to investigating the effects of mycorrhizal fungi species on salt tolerance of safflower var Koseh. A factorial experiment based on randomized complete design with 4 replications was performed in the pot under natural conditions in 2014. Treatments were four water salinity levels (0(control), 50, 100, 150 Mm NaCl) and five mycorrhizal levels (non-inoculated and inoculated with four species, Glomus mossea , G. intraradicea , G . versifrom , G. langula ). Plant height, leaf area and number per plant, proline, relative water, carotenoid, chlorophyll a, b and total chlorophyll contents, root and shoot fresh and dry weights, percent colonization, membrane stability and seed yield were determined. Salinity stress reduced leaf area and number per plant, relative water, carotenoid, chlorophyll a, b and total chlorophyll contents, root and shoot fresh and dry weights, membrane stability and seed yield but increased prolin content and percent colonization. However, the effects were species-salt level-specific. Highest proline, relative water, cartenoid (with G. versifrom ), chlorophyll a, b and total chlorophyll contents, membrane stability, leaf number, shoot fresh and dry weights, root fresh and dry weights (with G. versifrom ), percent colonization (with G. versifrom ) and seed yield was recorded under saline and non-saline conditions when plants were inoculated with G. mosse a, while the lowest cartenoid, chlorophyll a, b and total chlorophyll contents, root fresh and dry weights, shoot fresh and dry weights (with non-inoculated) was recorded under saline and non-saline conditions when plants were inoculated with G . i ntraradicea . Wheareas, the highest leaf area index was recorded under saline and non-saline conditions when plants were inoculated with G . versifrom . While the lowest leaf area index (with non-inoculated), proline, membrane stability and seed yield was recorded under saline and non-saline conditions when plants were inoculated with G .langula . Also, the lowest relative water content was recorded under saline and non-saline conditions when plants were not inoculated and the lowest leaf number was recorded under salin and non-salin conditions when plants were inoculated with G. versifrom . The results showed that inoculation with mycorrhizal alleviated salt tolerance in safflower. However, the effectiveness was depend on salinity level and mycorrhizal species and G . mossea and G. versifrom species were the most effective. Key words : Mycorrhizal fungi, salinity, physiological traits, safflower