Perovskia abrotanoides Karel is a medicinal plant with different applications in traditional and modern medicine. The genus of Perovskia belongs to Lamiaceae family. This plant is distributed in Iran, Afghanistan, Pakistan and Turkmenistan. It grows wild in natural habitats of Northern, North-Eastern, South-Eastern and Central regions of Iran. Study of inter and intra population diversity can provide new insights on the evolutionary processes and for the establishment of effective and efficient conservation strategy for small and endangered populations. In this study, 64 accessions from 16 populations of P. abrotanoides were collected from different geographical regions of Iran including Center, North, North-East and South-East. Furthermore, four accessions from one population of P. atriplicifolia were included in this study. So, the molecular and morphological characteristics were studied on 68 accessions using 13 ISSR primers that produced 148 bands in which 110 of them were polymorphic. Relatively high polymorphism (74.32%) obtained for ISSR primers in studied Perovskia accessions. Hierarchical cluster analysis (HCA), principle coordinates analysis (PCoA) and analysis of molecular variance (AMOVA) was performed for molecular data. Results revealed that genotypes were ). Molecular and morphological results did not confirm each other. Results of morphological analysis Kkeywords : P. abrotanoides , ISSR, Morphology, Genetic diversity
