The oil seed crops are economically important, because of their big contribution in providing the energy for both human and livestock. The increase of demand for vegetable oils in the world market have caused to pay more attention to oilseed production and expanding the research programs for these crops. Water deficit stress is an important factor to limit the production of the field crops in many areas of the world, particularly in arid and semi-arid areas. This study was conducted to evaluate 25 sesame genotypes separately at two irrigation regimes based on the MAD 60% and 90% for normal and stress condition, respectively. The experiments were conducted at the Research Farm of Isfahan University of Technology and a randomized complete block design with three replications was used for evaluation of the genotypes in each irrigation regime. The results of analysis of variance showed that there were significant differences among the genotypes for all of the studied traits, except for days to emergence, membrane stability index and relative water content in normal irrigation. However, in stress condition, the genotypes showed significant differences for all studied traits, except for emergence. The results of combined analysis of variance showed that the irrigation regime significantly affected on all of the traits that after irrigation regimes were measured. Also, the interaction effect between genotypes and irrigation regimes was significant for the traits of capsules per plant, seeds per capsule, seed weight, seed yield per plant and seed yield per plot. The range of the average seed yield per plot for genotypes under normal conditions between 346/8 to 1933/3 kg/ha (average 1032/2 kg/ha) and in limited irrigation regime between 262 to 1367/3 kg/ha (average 676/1 kg/ha) was variable.The correlation coefficients showed that seed yield per plant had significant and positive correlation with capsules per plant and seed weight per capsule in both moisture conditions. Genetic and phenotypic coefficient of variations showed that there was high genetic variation for most of the traits in both irrigation regimes, especially in stress condition. Moreover, relatively high broad-sense heritability was estimated for traits of branches per plant, seeds per capsule, seed yield per plant, seed yield per plot in both irrigation regimes. These results indicate that selection can be effective to improve these agronomic traits and to develop drought tolerant genotypes. The stepwise regression showed that capsules per plant and seeds per capsule significantly contributed in 63% of the variation for seed yield per plan in each of irrigation conditions. The results of path analysis also revealed that in both irrigation regimes, capsules per plant had the highest direct and positive effect on seed yield per plant. Generally, it seems that capsules per plant can be considered as an appropriate selection criterion in breeding programs of sesame to improve seed yield and developing drought tolerant cultivars. Key words : Sesame, Moisture regimes, Drought stress, Seed yield, Yield components