In urban agricultural systems, the main issue is choosing the suitable light source and its appropriate management. On the other hand, one of the most influential factors on growth and plant secondary metabolites is the intensity and quality of light applied. Therefore, using plant vertical farming has many advantages which can lead to guaranteed increase in crop quality and quantity with controlling and management of light. Novel LED technology, accompanied with modern greenhouses, has increased production and its efficiency in fruits, vegetables and medicinal plants and also has provided the condition for optimization of light-dependent processes of crop production. Also, monochromatic LED lights can increase the content of plant secondary metabolites. The two species of Stevia ( Stevia rebaudiana ) and basil ( Ocimum basilicum ) are among Lamiaceae medicinal plants. In this study, the effect of LED lights on morphological, physiological and biochemical characteristics of two genotypes of stevia and six genotypes of basil was evaluated in two separate trials according to a completely randomized design with tree replications and in a pot experiment. The light treatments were 5 light environments including 4 LED environments of red, blue, red-blue and white LEDs and natural light (greenhouse). The LED incubators were fabricated in four separate cabinets with one of 100% red light, 100% blue light, 70%red-30%blue combinational light or 100% white light. The light intensity was adjusted on 300 ?Mol photons m?2 s?1. The results showed that the performance of different genotypes was not the same in different LED environments. In Stevia, the highest fresh and dry weight of shoot was observed under red light and the highest concentration of chlorophyll and carotenoids were measured under red-blue lights. Also, under red light, significant increase in stevioside and rebaudioside content was detected. In contrast, in basil, fresh and dry weights were increased under red-blue LED lights and the highest content of phenolics and flavonoids were observed under blue and red LED lights. Among the genotypes, purple genotypes like Mobarakeh-purple were superior in terms of growth and productivity compered to their green counterparts. For the most morphological characteristics, basil genotypes have the highest phenotypic and genetic variation under red-blue LEDs indicating the highest level of genotype by environment interaction under this LED environment. Therefore, selection of the best genotype is performed better under red-blue combinational light than the other environment and greenhouse condition. Based on the bi-plot analysis, the best combinations of basil genotype and light environment in terms of growth and productivity were Ardestan-purple grown under red LED light and Mobarakeh-purple grown under red-blue LEDs. Regarding plant quality, the content of sweetener, stevioside, in Stevia and the flavonoid, rutin, in basil were highly expressed and accumulated under red LED light. In contrast, many phenolic compounds were not expressed in plants grown in greenhouse. It seems that both quality and quantity of plants have been improved under LED lights compared to greenhouse cultural condition. Key words : Stevia, Basil, Artificial light, LED, Flavonoid, Interactions