There is limited information on the effects and requirements of specific fatty acids for dairy calves. There are only 2 essential fatty acids (C18:2 and C18:3), yet these essential fatty acids have been shown to elongate into other functional fatty acids (C20:4, C20:5, C22:6, i. e) that are important for neural development and production of hormones. In previous studies specific fatty acids incorporated into milk replacers and growers have reduced scouring and improved ADG, feed efficiency, and hip width change in calves. Also, added fat has been reported to reduce intake and ADG in calves. Because, there is limited data on the effect of Linoleic acid on calf performance ,We hypothesize were to investigate the effects of feeding Safflower and Canola seeds to the starter diet on immune system and performance of Holstein dairy calves. In trial 32 Holstein bull calves that were fed 1 of 4 treatments and 8 treatments in every repeat. Treatments containing A) no Safflower seed and Canola seed (control), B) 5% Safflower seed, C) 5% Canola seed, and D) 2.5% Safflower and 2.5% Canola seed. In order to make better palatability Safflower and Canola seeds in digestive system, we did process on them. In this case, at first these seeds were heated and then grained them and added to initial diet. The starters were formulated to be equal in CP and energy. Calves received milk in trial fixed and equal to 10% of BW/D (half in the morning and half in the evening). After birth, all calves were fed enough amounts of Colostrums daily for the first 4 d, and calves were individually penned. Calves received every day feed, Dry feed offered and feed refusals were weighed weekly. Every calf consumed 1kg diet/day for three days continuously, and then was weaned. Calves were weighed weekly before the feeding and fecal scores and health score were recorded daily. In order to immune system stimulation with Sheep red blood cell (SRBC) injection was occurred twice (21 and 31 days after birthday) and then bleed blood samples of calves 10 days after injection (31 and 41 days after birthday). Data from trial were analyzed using SAS statistical software and the MIXED procedure as a completely randomized design, and initial body weight was considered as a quvariate variable. for calves was considered Time period three (1 to 3 weekly, 4 to 7 weekly and 1 to 7 weekly). In this experiment, there were no differences significant among calves treatment the control diet and calves other treatment at feed intake, feed efficiency, high body, around chest, than Albumin to Globulin, health score and fecal score. Average daily gain (from 21 to 49 day, p 0.01) weaning day and test antibody titer (p 0.05), of calves were improved in treatment Safflower compared with calves fed the control. This experiment shown that using Safflower seed at cause has essential fatty acid (C18:2) can as way suitable for improvements performance in calves. Keyword : Oil Seed, Canola, Safflower, Calf