Beside of nutrition, genetic potential is affecting on performance of production traits. Nutritional genomics, as a multidiscipline branch in science, says which animal may genetically be have the best feed efficiency. In the present study, the effect of Threonine dietary levels on expression of Interleukin-8 and interferon-alpha gene was studied in Arian broiler chickens. Based on a completely random design, six levels of Threonine (0.66, 0.76, 0.86, 0.96, 1.06, 1.16) were tested in starter diet (0 - 21 days) for 720 Arian broilers in 4 replicates and 30 individuals in each replications. All chickens were slaughter at age of 21 days and sampled from intestine (gegunom). Total RNA was extracted and then cDNA synthesized. The relative gene expression was estimated as difference from a housekeeping (reference) gene that is GAPDH using realtime PCR method. The ?cts were statistically analyzed by a generalized linear model using R software. The results showed that the Threonine levels were statistically affected on expression of two studied genes. The IL-8 and INF-alpha genes are up and down regulated compared to reference gene, respectively. The relative quantification indicated that of the need for the control group, it was so Threonine with 0.96% level IV patients as measure gene expression was used. In diet fed for chicken contains 0.86% Threonine in IL-8 gene, the most significant difference was observed in the intestinal membrane and rations containing gene expression in most routine% Threonine interferon-alpha view. Compared to contol treatment that considered as , relative quantification indicated that expression has been statistically increased in intestinal membrane at 0.86 and 1.06 treatments for IL-8 and INF-alpha, respectively Key Words: : gene expression, Threonine, Arian broiler, Interluekin-8, Interferon-alpha