The aim of this study was to evaluate the effcts of different levels of barley malt extract (BME) as a nutritional supplement on the performance and immune response of laying hens at the peak and after peak production. This experiment was performed with 432 laying hens in a randomized complete design with 3 treatments of 6 replicate each for 18 weeks. Three dietary treatments including 0%, 0.2% and 0.4% of barley malt extract were used. Egg production, egg mass, egg weight, feed intake, feed conversion ratio, shell thickness, shell strength, shell weight, yolk height, yolk color and Haugh Units(HU), as well as antibody titers SRBC, Newcastle and Influenza viruses and the height of the villus and the depth of the crypt in the jejunum section were measured. The results showed that the percentage of egg production, egg weight, egg mass, feed conversion ratio and feed consumption at the age of 28-46 weeks (peak production and post peak) were not affected by experimental treatments. The egg shell thickness (p 0.001) and shell strength (p = 0.07) increased in both malt extract recipients as compared to the control treatment. The internal quality of the eggs such as the Haugh Units (p 0.05) and yolk height (p 0.01) increased in the malt extracts treatments as compared to the control treatment. Production of primary antibodies against SRBC was affected by barley malt extract, and increased dietary supplemented with 0.2% and 0.4% barley malt extract (p 0.05). Primary production of IgG antibodies against SRBC increased in the groups supplemented with 0.2 and 0.4% barley malt extract (p 0.01). Production of antibodies against Newcastle and Influenza viruses was not influenced by experimental treatments. Villus height and crypt depth were not affected by the treatments, while villus height to crypt depth ratio was improved (p 0.05) by adding 0.2% BME to the diet. The results of this study showed that adding malt extract to the laying hens diet can have a positive effect on egg quality, immune responses and intestinal health in laying hens. Keywords Barley malt extract, Immune System, yield, egg, laying hens.