The present research was carried out for preparation of mesoporous starch (MPS) and schiff- base aerogel as carriers for genistein, a model of poorly water-soluble phytoestrogen isoflavone; and exploration of the impact of different fabrication parameters on structural and loading properties. MPS is considered as a highly porous biomaterial which typically possesses nanometer-sized porous microstructure and low density, providing a large effective specific surface area (SSA) and hydrophilic surface to improve solubility, stability and bioavailability of poorly water-soluble active agents. To fabricate MPS, various concentrations (8-14% w/v) of starch from different sources (corn, potato and tapioca) was used for gel formation and the successive solvent exchange process was performed with use of various ethanol concentrations (40-70% v/v), which were then dried by different techniques (rotary vacuum evaporation, microwave and freeze drying) . MPS quality attributes such as SSA, total porous volume, BJH pore diameter, swelling ratio, angle of ripose and compressability index were determined and effects of the fabrication parameters were investigated using L9-Taguchi orthogonal array design. The results indicate that second order polynomial regression models were well ?tted for all response variables. Interestingly, the starch components greatly in?uenced physical properties of MPS. Also, the drying type and ethanol concentration altered signi?cantly the model equations. The overall best fabrication condition (14% corn starch, 100% ethanol concentration in ageing step and rotary vacuum drying) resulted in favorable MPS preparation with mean size of 105.4 nm and unimodal distribution. In the next step, genistein was encapsulated in MPS microstructure at different ratios, resulting in high loading capacity and efficiency (44.71% and 79.9%, respectively ) at 1:1 weight ratio. According to the electron microscopy and XRD analysis, the degree of genistein crystallinity lowered remarkably after the impregnation in to MPS, indicating improved solubility. In-vitro release profile of genistein from MPS in the simulated gastrointestinal buffer solutions (pH 1.2 and 6.8) demonstrated that incorporating genistein into the MPS enhanced the dissolution rate compared with genistein powder. Release kinetic data were fitted to the Higuchi model (R 2 = 0.98), indicating diffusion controlled-release mechanism. In the following, for impromvment of muccoadheseive property of carrier and control release of genistein, amorphous Schiff- base aerogel was prepared from interaction of dialdehyde starch and carboxy methyl chitosan trough imine bond (according to FTIR and XRD analysis) at different weigh ratio. Dialdehyde starch was prepared by oxidation of starch with sodium periodate (1:1 mole ratio) with 62.35 % aldehyde groups. Work of Mucoadhesion of Schiff base aerogel (1:1 polymer ratio) was significantly (about 4 times) greater than MPS and with increase of CMCh concentration was increased. In the next step, genistein was encapsulated in Schiff base aerogel microstructure at different ratios, resulting in high loading capacity and efficiency (43.8% and 77.95%, respectively ) at 1:1 weight ratio. The experimental genistein adsorption data were found to be fitted well to the Langmuir model (R 2 = 0.989) in both carriers. In-vitro release profile of genistein from Schiff- base aerogel in the simulated gastrointestinal buffer solutions (pH 1.2 and 6.8) demonstrated that release of genistein was pH dependent (greater in pH 6.8), slower and in controlled manner compared with genistein release from MPS (according to MDT DE). Release kinetic data in pH 1.2 and 6.8, were fitted to the Higuchi and korsmeyer peppas models, repectively. Antioxidant activity of genstein was evaluated trough ABTS + scavenging ability. Incorporation of genistein in Schiff- base aerogel significantle caused increase in scavenging ability of genistein (about 2.3 and 1.42 times greater than genistein in ethanol and genistein in MPS, respectively). Finally, loaded genistein in schiff- base aerogel was used for enrichment of tortilla (100mg genistein/100g tortilla) and to evaluate its stability trough tortilla preparation steps. Stability of genistein significantly increased by incorporating in Schiff- base aerogel (89.17% genistein recovered after tortilla prepation). In addition, ABTS + radical scavenging activity of genistein loaded Schiff- base aerogel in tortilla was about 15.72% greater than genistein powder. Altogether, well-tuned MPS and Schiff- base aerogel fabrication method can be utilized for an efficient encapsulation, dissolution enhancement and controlled release of poorly soluble phytochemicals, such as genistein.