Phytoplasma caused diseases pose a potentially serious threat to the production of fruit trees worldwide and cause great losses of these products. In recent years, typical symptoms of phytoplasma induced disease on apple trees including witches’ broom, leaf stunting and abnormal growth of petioles and also rolling leaf, quick decline and slow decline of pears, have been observed in different areas of Isfahan province. To detect and justify; MARGIN: 0in 0in 5pt; mso-line-height-alt: .9pt; mso-layout-grid-align: none" dir=rtl Key words : Apple, Pear, Phytoplasma, Apple proliferation, Nested-PCR, PCR-RFLP, DNA sequencing