Thedifferent aspects of using dietary supplements such as prebiotics inaquaculture and their effects on innate immune response and especially theirvertical transmission are of a grave questions. To address such this questionin both horizontal and vertical transmission of boosting immune system, thepresent study was designed to investigate the effect of different levels ofdietary Agrimos ® on the innate immune-related gene expression(lysozyme ( lyz ) and tumor necrosis factor alpha ( tnf-? )), andalso growth hormone ( GH ) gene expression, DNA methylation and threehistone methyltransferase [HMTs (H3K4, H3K9, H3K27)] activities in zebrafish ( Daniorerio ). Three hundred and sixty healthy 24-days-old zebrafish were randomlydistributed in twelve aquaria assigned to four groups. Zebrafish were fed witheither control diet or a diet supplemented by different levels (2, 4 and 8 g.kg -1 )of Agrimos ® for 90 days. The offspring of each treatment wasassessed to find the potential of vertical transmission of immunity by usingthis dietary prebiotic. At the end of the experiment gene expression studiesrevealed significant up-regulation (P?0.05) of tnf-?, lyz and GH genes in 2, 4 and 8 g.kg -1 Agrimos ® fedfish compared with control group. Although our findings showed thatsupplemented diet-reduced DNA methylation (P?0.05) inAgrimos ® treatments compared with control, there was no significantchange in all three HMTs’ activities among experimental groups (P 0.05). Theresult shows the successful transmission of lyz gene expression as aninnate immune response to the offspring of the treated adults and supports adirect role of DNA demethylation in the regulation of these candidate geneexpressions, suggesting possible role of diet on regulating the epigeneticprocesses. Increasingthe number of villi and goblet cells in fish treated with agrimimus alsoindicated a positive effect of this supplement on intestinal tissue protection,increased nutrient uptake, and increased nutritional efficiency. An increase inlactic acid bacteria was also observed in fish intestine of prebiotictreatments, and these results generally indicate the effect of nutritionalconditions on immune and growth factors. Keywords: Prebiotic;Agrimos ® ; innate immunity; gene expression; epigenetic; zebrafish.