This study aimed to evaluate the effects of dietary bovine lactoferrin (bLF) on growth parameters, physiological condition, iron metabolism and non-specific immunity in diploid and triploid rainbow trout. For this purpose, four different concentrations of bLF(0, 100, 200 and 400 mg/Kg diet) were supplemented in the diet in triplicates and fish (initial mean body weight = 3 g) were fed for 8 weeks. Dietary bLF didn’t affect growth performance (final weight, condition factor, weight gain, specific growth rate and feed conversation) in different groups of diploid and triploid fish. However, the hepatosomatic, visceral and visceral fat indexes showed significant differences between diploid and triploid groups (P 0.05). Main hematological parameters (red and white blood cells counts, heamatocrite, hemoglobin and differential white blood counts) didn’t vary between diploid and triploid one. Dietary bLF didn’t change serum total protein, albumin, globulin as well as hepatic enzymes between experimental groups. The plasma iron and total iron binding capacity were not affected by dietary bLF among groups. However, bLF had significant effect on serum triglyceride and cholesterol levels in triploid fish, where fish fed on the higher level of bLF showed lower plasma levels of these lipids. bFL inclusion in the diet could significant reduction in muscle iron contents while iron concentration in the liver were increased in both diploid and triploid fish; However, the effective dietary levels of bLF varied between diploid and triploid trout.Carcass qualities (protein level in triploid fish as an exception) were not affected by dietary bLF. The bLF supplementationcould improve non-specific immunity parameters, serum complement activity and lysozyme levels in diploid and triploid fish, respectively. As final results, both diploid and triploid trout showed similar physiological responses (immunity as an exception) to inclusion of bLF in the diet. Keywords : Salmonids, Chromosome manipulation, Non-specific immunity, Growth.