Some 223 specimencs of trout barb ( Capoeta trutta ) from four rivers of Bushehr Basin and eigth rivers of Tigris Basin were cought by seine net to compare their morphology using geometric morphometrics. The specimens, after collection, were fixed in bufferd 10% formalin and transferred to the Fisheries Laboratory of Isfahan University of Technology for further studies. Three morphometric and 14 meristic features, body weigth, age, as well as geometric morphometric method were applied to accomplish the biometric studies. Besides, some biometric features including length-weigth relationship, condition factor and growth performance index were calculated. For statistical analysis, the Kolmogrov- Smirnov Analysis, One Way ANOVA, Duncan groupins, Kruskal–Wallis analysis, Canonical Variable Analysis (CVA), Principle Component Analysis (PCA), Cluster Analysis (CA) and Discriminant Function Analysis (DFA) were employed. The PCA, CVA and CA showed differences between KheirAbad and Fahlian populations from Bushehr Basin and among Konjancham, Doirej, Sirvan, Zimakan, Karun, Karkheh and Direh from Tigris Basin in geometric morphometrics. Total length, Fork length, Standard length, number of lalteral line scales and number of predorsal scales, number of anal, dorsal, ventral, pectoral and caudal fins soft rayes, number of scales above and below the lateral line and circamucaudal, age and body weigth were diferent (p 0/05) among Bushehr Basin populations. Total length, Fork length, Standard length, number of anal, dorsal, ventral, pectoral and caudal fins soft rayes, number of ventral and pectoral fins hard rays, number of scales on, above and below the lateral line and circamucaudal, age and body weigth were diferent (p 0/05) among Tigris Basin populations. This result showed that traditional and geometric morphometric methods could separate many populations of Bushehr and Tigris Basins from each other to great exrent. However, for more details, molecular studies are needed. Key words: Biometry, Carp fish, Geometric characteristics, Meristic