The effect of zinc and copper supplements in napli of Artemia was studied on growth rate, survival, body composition and skeletal malformations of tilapia larval ( Oreochromis niloticus ). The enrichment napli of Artemia was based on the results, obtained concentration of zinc and copper (EC 50 ) and the optimal time of harvesting Artemia napli. The effective concentration of zinc and copper in the case of Artemia napli were (4.797 -6.07-0.78) and 0.44 (0.364-0.526) 0.44 mg / L, respectively, the optimal time of harvesting Artemia napli was 23 hours after incubation were considered. The enrichment concentration for each of both metals were 10 and 20% for EC 50 . The accumulation of Artemia napli under the influence of zinc and copper in terms of EC 50 , containing copper (copper ion using copper sulfate salt) in two levels of 69.2: 69% Cu and 74.9: 20% Cu micrograms per gram of dry weight of napli and zinc (zinc ion with zinc chloride) were calculated at two levels of 80.27: 10% Zn and 30.8: 20% Zn micrograms per gram of napli dry weight and control group (without adding zinc and copper). Combined treatments of zinc and copper were considered as a 2×2 factorial. 5 day larvae with an average weight of 0.177±0.055 g and a total length of 125.2±3.2 mm in 9 groups of 50 each. Three servings per day were feed by enriched Artemia (control group received without enriched Artemia napli). After 7 days of feeding larvae with Artemia napli, 30 larvae were randomly collected from each group and in order to measure the growth performance, the accumulation of zinc, copper, calcium, phosphorus and magnesium and dye Elysium blue and Alizarin red were used. Based on the results of this study, different levels of zinc and copper had a significant effect (P 0.05) on body weight gain compared to the control group. The highest standard length observed in Cu2 Zn1. Accumulation of zinc and copper in the body of tilapia larval increased in different treatments of zinc and copper (P 0.05), which indicates of the effectiveness of enrichment in the preservation of rare elements in tilapia fish. The highest copper accretion was observed in Cu2 Zn1 treatment and the highest accumulation was observed in Cu2 Zn2 treatment. In combination treatments, increasing the amount of zinc resulted in a decreasing trend in the amount of copper, while copper levels did not significantly affect the accumulation of copper. Used of zinc and copper supplements causes an increase in the content of calcium, phosphorus and magnesium in the larvae in single treatments and a significant increase (P 0.05) in the combined treatments. The results of this study showed that used of zinc and copper supplements can result in a better absorbed of the elements and improve the growth performance in this species. Key word: Enrichment, skeletal mineralization, copper, zinc, Artemia napli, Tilapia