Aquatic systems are dynamic and stable systems which are important in terms of biodiversity and geography. One of the most important biological habitats of zooplanktons is that they have high biodiversity and are highly biological, ecological and aquatic. Cladocera is one of the most important zooplankton of fresh water, which can reflect the biological conditions and is usually important in ecological studies. In this study, two species of Ceriodaphnia quadrangula and Daphnia magna were cultured to induce resting egg, molting, production of neonates, and determination of the amino acid in laboratory conditions by temperature manipulation and the light/ dark conditions. In the first experiment, the conditions for shock were 0, 32 and 0-32 for one hour and one treatment without shock. The temperature shocks were taken with light and dark at a light intensity of 100 mol photons/ m2/ s for a period of 12 days in the second experiment. Each of the experiments was performed in a completely randomized design with three replications. Feeding was performed with micro algae Scenedesmus quadricauda at a concentration of 5 × 106 cells/ ml. The amount of amino acids of two species was measured by HPLC. The highest amount of the neonate production and molting in the C. quadrangula was in the treatment of light and temperature shock. The average of neonate and molting production in D. magna were not significantly different from those of temperature shock and temperature shock together with light and dark. Shock applied to C. quadrangula and D. magna had no significant effect on ephipium production. The total amount of essential amino acids C. quadrangula (16.96 g/ 100 g) is less than D. magna (18.08 g /100 g), and the total amount of unnecessary amino acid C. quadrangula (17.03 g /100 g) One gram in 100 grams) is greater than D. magna (16.34 g/100 g). Keyword : Water flea, Ceriodaphnia. quadrangula, Daphnia magna, temperature, shock dark/ light period, amino acid composition