Investigating damages caused by wild species to the livestock, poultry, farms and humans are important issues in wildlife management. The first step in preventing damages, is to identify factors that play important roles in conflict between humans and wildlife. In the east of Isfahan Province (Ardestan and Naeen regions) there is a conflict between the local community and carnivores, which have been caused numerous financial, psychological and psychological damages to people. In the current study, 367 individuals of the local inhabitants from 18 villages were selected and interviewed. Further, the damaging species and the amount of vulnerability of livestock and poultry due to disease and the attacks of carnivores were explored. Results showed that about 92.7 percent of the livestock attacks and losses caused by grey wolves, and other species including jackal, common fox, hyena, wildcat, caracal and panther were responsible for the rest. Generalized linear model(GLM) was used to assess the important factors affecting the human and the wolf conflict and investigate local community's attitude towards the grey wolf. The correlation between local attitude toward the gray wolf and the age and education of people were investigated and factors affecting the number of livestock death were modeled. The number of dead cattle was significantly correlated with the number of immature individuals, place of attack, the height of the stable, the number of sheepdog, the duration of animal husbandry activities, the material of the stable (brick, cement, and block), the time of attack (day or night) and the existence of projector or fence. The number of incurred fatality by other factors such as disease was 2.5 times greater than the losses due to the wolf attacks. Perhaps the local people encounters with the gray wolf and its aggressive behavior may have been responsible for the negative attitude of people towards the gray wolf. It seems that the restriction of sheep and other livestock in roofed cement and block corrals which cared by sheepdogs and avoiding the places where wolfs are abundant, especially during the wolf breeding and nursing seasons, are the most reasonable ways of decreasing the human –wolf conflict. In fact, the conflicts can be decreased but cannot be totally eliminated. Key words: the number of died livestock, gray wolf, Isfahan province, local community's attitude, Generalized linear model(GLM).