The response of plant species to the ecological factors is an efficient guide in improvement, restoration and harvesting projects of range lands. Present study was done in order to investigate some ecological characteristics of Hedysarum criniferum Boiss in five separate stages. In the first stage, the effects of environmental factors on distribution of H. criniferum was studied in Chadegan and Mute region in Isfahan province. A total of 30 soil samples were collected from the 80 randomly established plots. Canonical Correspondence Analysis was used to explore the relationships between environmental and management factors with the species distribution in CANOCO software and Cluster analysis was also used to group sampling plots. The results showed that the presence of the species is mainly on loamy, sandy loam and silty loam soils. In Chadegan habitat, this species appear more in the North and East slopes with convex shape and in Mute mostly scattered in of North slopes. Unlike some other species in these regions, H. criniferum is not sensitive to soil calcium and magnesium. The most presence of H. criniferum was seen in the low gravel percentage (0-10%) and in 60-80% slope. In the second stages, the effect of pollinators and seed predators on regeneration of H. criniferum was investigated. To achieve the goal, the tulle-mesh cages with rebar in the dimensions 75×150×150cm were used to apply the following treatments: pollinators excluded/seed predators present; seed predators excluded/pollinators present; seed predators excluded/pollinators excluded; or control (neither excluded). The GLM procedure was implemented to evaluate the set of data in 2×2 factorial design in completely randomized design. The results show that the pollination impact is supportive and causes increment of 47.6 fold of loments and 62.6 fold of produced seeds compared to their absence. In addition, the seed predators infected 27% of the seeds compared to the control. Therefore, it was found that the pollinators are more effective than the seed predators on the reproduction of this plant. In the third stages, the germination characteristics of H. criniferum were studied in different temperature and moisture conditions in vitro. The experiment was conducted as a randomized complete design with two factors (first factor included four alternative temperature levels and the second factor includes four drought levels) with three replications. Based on the results, as the temperature and drought levels increase, the germination percentage and germination speed of the species decrease. Incredibly, the seeds even germinated in high osmotic potential (-6 bar). Higher germination percentage of H. criniferum seeds in different drought levels in comparison to high alternative temperature (24-26 °C) indicated that this species is more sensitive to higher temperature than high levels of drought condition. Therefore, it could be concluded that the H. criniferum is a relatively drought resistance species. Some indicators of quality forage of H. criniferum in different phenological stages were determined in the fourth stages of this research. In all phenological stages, in Muteh the high amount of ADF, NDF and CF as reducing agents of forage quality was observed and conversely, the high amount of CP, TDN, DMD and ME as the increasing forage quality was detected in Chadegan. This species is “highly desirable” regarding the rangeland forage quality normal" H. criniferum , Astragalus cyclophyllon and Bromus tomentellus (non-legume plants as control) as well as the control soil at 0-30 and 30-60cm depths in a completely randomized design. Results showed that the amounts of inorganic nitrogen, total nitrogen and organic carbon at 0-30cm soil depth were significantly higher than the other treatments and were decreased with increasing in depth. In general, regarding the importance of this native legume, its preservation and conservation of this valuable resource within the optimal management framework seems a must. Key Words: Autecology, pollinator insects, seed predator beetles, germination, forage quality, nitrogen fixing