Arid and semi-arid ecosystems are degraded in some extent around the world the reclamation of these ecosystems is challenging due to their fragility, size and expenses. Soil condition can be changed permanently if pressure on vegetation condition continues; hence ecosystem can not reverse to previous condition and may leads to desertification. Evaluation of landscape structure and function provides scientific understanding to support sound management and conservation in rangeland ecosystems. Landscape Function Analysis (LFA) is introduced as a quantitative assessment approach to evaluate functionality of ecosystems in Australia and was widely used in many other ecosystems. In this method eleven indicators are being estimated in patches and inter-patches along a 30 meters transect to obtain three indices including stability, infiltration and nutrient cycling. This study aimed to verify LFA accuracy in arid and semi-arid rangeland of Iran. Also the LFA indices were compared in protected and unprotected sites. Fourteen sites were selected in different climatic zone in Isfahan province (7 sites in semi-arid zone in Bardasaian and Aghagol region and 7 sites in arid zone in Soh and Muteh). The LFA indices measured using field studies and some properties of surface soil samples were studied in laboratory both for different patch and inter patches. Soil texture, mean weight diameter (MWD), organic carbon% (OC %), CaCO 3 %, sodium adsorption ratio (SAR) and electrical conductivity (EC) of all soil samples were measured. The correlation analysis was conducted between two LFA indices (stability and nutrient cycling) and MWD and OC % data. Also LFA indices of protected and unprotected sites were compared using one way analysis of variance and LSD ? s mean. The correlation of direct measurements data (results obtained from MWD and OC %) with indirect measurements data (soil stability and nutrient cycling index obtained from LFA) of all sites were significant except one site in Soh region. According to the results, function indices were much higher in patches than inter patches (bare soil). Also LFA indices of different patch types and the whole sites varied significantly in some sites. Function indices were decreased as grazing intensity increased in Bardasiab and Aghagol regiosn. According to results, Stability index of protected area in Soh was significantly lower and its infiltration and nutrient cycling index were higher than unprotected sites. The main reason for low stability index in protected area of this site was the fact that sand sediments were aggregated by patches. There was significant differences.(P 0.05) between LFA indices of patches located in arid and semi arid zones. Only nutrient cycling was significantly different (P 0.05) when inter- Keywords: Stability soil, Organic matter, Function indices,Rangeland, arid and semi-arid ecosystem