Vegetation composition and their cover are representative of climate, soil, topography a specific areas. Hence knowledge of environmental factors influencing on the distribution of species and vegetation communities is important, It is essential to determine change of vegetation communities along environmental gradient as well as indicator species response curve to environmental variables in rangeland ecosystems. According to geographical locations and ecological conditions, we selected five rangeland sites to investigate relationship between vegetation with some habitat characteristics in Fereydoun region of Isfahan province and determine the effective factors on establishment and growth of plants along environmental gradients. The most important characteristics of ecological sites in three areas i.e. climate (13factors), soil, physiographic (13 factors) and vegetation (15 species), were determined using systematic-random method. Climatic factors were estimated by simple linear regression models of five meteorological stations. For each site, soil profile was excavated and some physical and chemical properties of soil were measured. Elevation, slope and aspect were studied using topographic maps of 1: 50,000, inclinometer and GPS. A factor analysis was performed to reduce number of environmental variables by software v. 22 and 19 environmental factors of 26 factors were selected. Quantitative values for 19 environmental factors were clustered based on relative Euclidean distance and minimum variance method to assess similarities between studied sites. According to the results of cluster analysis, two habitats distinguishable at similarity level 87.5 percent. The first habitat included sites (1, 2, 4 and 5), second habitat is included sites (2 and 3). Principal components analysis was used to investigate relationship between plant composition cover of 15 plant species and ecological conditions including nineteen environmental factors. Results of PCA demonstrated that Astragalus gossypinus is dominant species in first habitat . Some environmental factors such as drought conditio and high fluctuated temperature (average min and max temperature 4.6 and 16.8 °c respectively) along with soil with low lime (CaCo3 37%) are responsible for isolation of this habitat from others. Stipa barbata is dominant plant species in second habitat (including sites 2 and 3) with intermediate climate and soil conditions. Astragalus adscendens type is dominant plant in third habitat (sites 4 and 5) characterized with more humidity and lower temperature (average min and max temperature 5 and 11.5 °c respectively), southern slope, heavy and deep soil and high organic matter. it provide more suitable condition to establish more plant species. Results of ordination analysis and cluster analysis on environmental factors are same too. Keywords: Principal component analysis (PCA), cluster analysis, Ordination, environmental factors, Fereydoun shahr City.