Nowadays, layered materials is one class of drug delivery vehicles that has received more attention and can accommodate therapeutic compounds between their layers. Recently more considered the use of layered silicates such montmorillonite that an aluminosilicate for desorption control system. Also, that is used because of unique and structural features such as high specific surface area, cation exchange capacity, colloidal properties, good adsorption, and the ability to increase interlayer space, non-toxic and low power potential allergens to solve medical problems. In study, the montmorillonite was purified with three different procedures in order to achieve purified nanoclay. For physical purification 2.5 mm particles of raw montmorillonite were collected using centrifuge force (2-CC). In the second method, 2.5 mm montmorillonite particles were reacted with 1 M HCl and then centrifuged (CCH). In the third method, 2.5 mm montmorillonite particles were reacted with 1 M NaOH and then centrifuged (CCN). Some impurities such as quarts and calcite have been removed of samples. In addition, the montmorillonite was modified with two method. The results showed that by increasing the pH the amount of adsorption of vitamin B12 reduced for all samples. Release of vitamin B12 from montmorillonite was performed at two different pH. Results showed that at pH 1.2 almost no amount of vitamin was released from any of montmorillonite samples. By increasing the pH from 1.2 to 7.4 the vitamin B12 started to desorbed and the desorption results at pH 7.4 for all samples. The highest amount of vitamin was desorbed for samples 2-CC and CCN when the adsorption was carried out at pH of 5 in which the dominant forces of adsorption was the electrostatic forces and by increasing the pH higher repulsion forces appears resulted in the higher amount of vitamin to desorb. In the next step, In order to specify the desorption mechanism of vitamin B6 from different adsorbent samples, the adsorption experiment for the duration of 60 minutes were performed. For 2-CC and CCN samples were detected the highest amount of desorption at adsorption pH of 9.5 because a lower concentration of H + groups excite on this sample and vitaminB6 is deprotonated form in high pH and higher repulsion forces are between 2-CC sample and vitamin