As global demand for zinc increases and the overall grade of its ores decreases; As well as the lack of response to common methods for these types of ores; Processing of this metal with new methods such as bioleaching and bio-heap leaching is necessary. In this study, the ability of bio-heap leaching for low-grade sphalerite soil of Bama mine, which includes high amounts of dolomite tailings and pyrite, was investigated. In these studies, mesophilic and thermophilic bacteria (sulfur and iron oxidizers) were used. The purpose of this study is to investigate the possibility of recovering precious metal from low-grade soil by bioleaching. The studies were first performed in the laboratory in a vibrating flask. In these experiments, first the parameters of temperature, acid consumption, type of culture medium, type of bacteria, amount of ferrous sulfate in the culture medium and the composition of concentrate and soil were investigated. The results of experiments with vibrating dishes showed that the best temperature for mesophilic and thermophilic bacteria is 35 and 45 degrees Celsius. The amount of acid consumption in this test is 7.29 grams per ton, they grow better in 9K culture medium and the required amount of ferrous sulfate is more than 10 grams per liter. In the composition of concentrate and pyrite soil, the recovery reached over 96%. Recovery of zinc metal was obtained by mesophilic and thermophilic bacteria 86 and 88. Both types of microorganisms showed better recovery at pH 1.8. These experiments paved the way for bioleaching isotherm columns. Several different parameters including particle size (12 and 21 mm), bacterial type and bacterial mixture were investigated. The dissolution rate of zinc increased with increasing amount of ferric ions. Bacterial activity increased with increasing redox potential. Iron oxidizing bacteria became active much earlier than sulfur oxidizing bacteria. The best particle size of 12 mm was the best leaching of thermophilic bacteria. In this system, in mesophilic bacteria with particle size of 21 and 12, zinc recovery was estimated to be 46% and 63%, respectively; 48% recovery for thermophilic bacteria with a particle size of 21 mm; And 91% recovery was achieved in the microbial and geoactive