In this work, Pt/rGO (40% wt.) as an anodic catalyst was investigated for the oxidation of glucose under the conditions near to the human body physiological conditions. The oxidation region of glucose was detected on the platinum surface in phosphate-buffered saline () at a potential of -0.2 V vs. Ag/AgCl reference electrode. Additionally, the stability of the catalyst against the glucose oxidation intermediates in containing 5 mM glucose was investigated over 400 cycles by a prolonged cyclic voltammetry study. Using a variety of amino acids and organic molecules with concentrations near to the physiological tissue fluid of the human body, their interaction with the glucose oxidation were investigated. The maximum glucose oxidation peak’s current in the hydrogen desorption region for alanine, aspartic acid, glycine, leucine, methionine, and urea was decreased by 42.5, 11, 90, 14.2, 5, and 55%, respectively. For ascorbic acid, cysteine, ??and isoleucine the maximum glucose oxidation peaks current in the hydrogen desorption region was increased by 39, 5.7, and 6.4%, respectively. The activity of the anodic catalyst for glucose oxidation in real human physiological conditions was also examined using human blood serum. Results indicated that the maximum glucose oxidation peak’s current in the hydrogen desorption region for the 50 th cycle in blood serum was decreased by 87.5% compared to the 50 th cycle in glucose solution. Moreover, after the poisoning effect of the studied amino acids and organic molecules, by a regeneration protocol, activity regeneration of the Pt/rGO was evaluated. Activity of the catalyst was recovered by 72.4, 91.2, 91.6, 98, 98, and 99% after poisoning by methionine, alanine, urea, cysteine, leucine, and glycine, respectively. Results indicated that the catalyst’s activity was completely recovered after poisoning by isoleucine, aspartic acid, and ascorbic acid. Lastly, the performance of Pt/rGO (40% wt.) as the anodic catalyst for glucose oxidation was investigated in a complete glucose fuel cell in search of an appropriate anode electrode for the abiotic implantable glucose fuel cells. containing glucose was used as the stationary fuel at both anode and cathode sides, with concentrations near to the physiological conditions of the human body. During 15 h of constant current discharge of 0.01 mAcm -2 (sufficient current density to power a pacemaker), the glucose fuel cell voltage was decreased by 23%.