During the last decades, population growth and increasing migration to urban areas has led to an increase in population density. The urbanization trends in Iran has resulted in degrading environmental quality in many parts of Iran, particularly in the metropolitan areas such as Isfahan. The recent changes in landscape pattern for development has modified the traditional landscape structure, increasing humanity to a new level of noise exposure that is considered a form of environmental pollution. Quiet areas are associated with relevant ecological values, and in national parks and wildlife refuges their value is compared to clean air quality. Soundscape ecology is the study of biological (biophony), anthropogenic (anthrophony) and geophysical (geophony) sounds produced from the landscape, their spatial and temporal variation, and their relationships with ecological processes and human activities. This study is the first effort in Iran to introduce the application of soundscape ecology concepts in urban planning and management. Here we examined the relationship between landscape pattern and the soundscape in an Urban-Natural gradient in Isfahan province, Iran. A series of landscape metrics (including NP, LPI, PLAND) were calculated using FRAGSTATS software, and acoustic surveys were conducted and compared in different land use/land covers in the study area. For the sound survey, 90 measuring stations were selected randomly (10 stations within 9 buffer zones around Hakim street, in Isfahan city center). Audio parameters were measured using Brüel and Kjayr, model.2230 device. Results of this study indicated that by moving away from urban areas toward natural areas, number of urban and natural patches decrease and increase, respectively. During this gradient, agricultural land showed as the highest number of patches at distance of 20 -30 km from the center of study area (Hakim Street). The variety of land uses increased up to the distance of 25 km and then decreased in the next buffers. The highest sound diversity was also measured in 25 km buffer. In urban areas, anthrophonies was at the highest level, while the most biophonies was calculated within natural areas in Sofe mountain park. The maximum sound pressure level which was 85 db was calculated in the airport surrounding area, and also in some parts of Isfahan city, Shahin shahr, Khomeini Shahr, NajafAbad, Dolatabad, Borkhar and Zarrin Shahr. The lowest sound pressure level was calculated at Kolah qazy and Qamishlo national parks. For mapping the noise pollution in study area the nearest neighbor interpolation with lowest RMSE, MBE and MEA value was considered as the best method for this study. Our results suggest that the land use/cover type and landscape structure is strongly associated with acoustic measurements, and this results in human-dominated landscape could be useful for urban and landscape planners to reduce anthropogenic noise and improve the quality of the soundscape. Keywords : Landscape patterns, Noise pollution, Soundscape ecology, Sound pressure level, Isfahan.