Poly(vinyl alcohol)/organo-modified Cloisite Na + bionanocomposites were prepared with various amount of organo-nanoclay. For this purpose, at first the pristine sodium montmorillonite (Cloisite Na + ) was modified by protonated of enantiomeric pure L-leucine amino acid as a cationic surfactant. Gallery spacing and thermal stability of this chiral organo-nanoclay were studied by using X-ray diffraction and thermogravimetric analysis, respectively. Also the bionanocomposite films were characterized by X-ray diffraction (XRD), fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and UV-visible transmission spectra. The poly(vinyl alcohol)/organo-modified Cloisite Na + bionanocomposite films showed improvement in their thermal properties. . In the second project, Poly(vinyl alcohol)/organo-modified Cloisite Na + /TiO 2 nanocamposites film were prepared with 10 wt% of organo-nanoclay and various amount of TiO 2 Cloisite Na + has been modified via cation exchange reaction using protonated form of natural L-leucine amino acid as a cationic surfactant. Then poly(vinyl alcohol)/TiO 2 /organo-nanoclay nanocomposites were synthesized by dispersion of TiO 2 on the surfaces of organo-nanoclay in poly(vinyl alcohol) matrix. In this process, nanocamposite films were prepared using ultrasonic energy following by solution film casting method. Three nanocomposites with different loading of TiO 2 (1, 3 and 6 wt%) were prepared and characterized by XRD, FT-IR, field emission type scanning electron microscope (FE-SEM), SEM, TEM, TGA and UV-visible transmission spectra. In the third project, surface modification and characterization of TiO 2 nano-particles as an additive in the poly(vinyl alcohol)/organo-modified Cloisite Na + nanocomposites with 10 wt% of organo-nanoclay were investigated. For the improvement of nanoparticles dispersion and increasing possible interactions between nano-particles and polymeric matrix, the surface of the nano TiO 2 is modified with silane coupling agent (KH550). In this study, the nanocomposites were prepared by ultrasonic process and following by solution film casting method. The novel nanocomposites were synthesized by immobilizing of modified TiO2 nanoparticles on the surface of organoclay layers. Three nanocomposites with different loading of modified-TiO 2 (1, 3 and 6 wt%) were prepared and characterized by FT-IR, XRD, TGA, TEM, SEM, FE-SEM and UV-visible transmission spectra. Morphological images observed through SEM, FE-SEM and TEM. The results show that the OMMT was dispersed homogeneously in PVA matrix and modified TiO2 nanoparticles are regularly dispersed on whole surface of organoclay layers.