Damask rose ( Rosa damascena Mill., Rosaceae) is one of the oldest rose species and important species as a source of rose oil and rose water grown in Iran. At present, this plant is cultivated throughout the world for ornamental, medicinal, culinary and fragrance purposes. Genetic variation of this species can help breeders to conducting the breeding programs. Molecular markers with capability to appear cytoplasmic inheritance and targeting of coding sequences can help to 0.09; P 0.001) with a fairly gene flow. Among 25 primer pairs used in cR analysis, 13 primers were amplified polymorphic bands with a number of two alleles per primer. The mean of PIC, the average values of Nei's gene diversity and Shannon’s information index were 0.1, 0.16 and 0.28, respectively. Dendrogram constructed using Jaccard’s similarity coefficient and UPGMA method in cR data was divided the genotypes into three main groups. The results of principal component analysis (PCA) were showed that the first three components accounted for 61.74% of the total variation, indicating that both of PCA and clustering method can be appropriate to study of the cR data. In addition, the data obtained from SRAP and cR markers were combined and the dendrogram was plotted by UPGMA method. This dendrogram clustered the genotypes in separate groups, as well as SRAP result. It can be concluded that SRAP is an effective marker system in compared to cR for studying genetic diversity and relationships among R. damascena genotypes and the results from SRAP marker were more corresponded to geographical distribution of R. damascena genotypes in Iran. Key words : Rosa damascena; Genetic variation; SRAP; cR; Iran
