The industrial, agricultural and urban activities have caused pollution of heavy metals in soil that can also increase concentration of the metals in food chains. Because of the heavy agricultural and industrial activities in province of Isfahan we need to determine the concentration of heavy metals in the soil. The purpose of this study was to determine spatial variability of Arsenic (As), Selenium (Se) and Vanadium (V) in Isfahan soils. In this research, the topsoil samples (0-20 cm) were collected in a stratified random sampling system at about 4 Km intervals in a study area of 6800 Km 2 . The positions of samples were recorded using a GPS. After preparing in laboratory, the soil samples were measured for total As, Se and V by a XRF, soil pH, organic mater ( OM ), sand silt and clay contents, soil electrical conductivity (EC). Spatial structures of variables were determined by variograms. Variograms were prepared in directional form for all the variables. The results show lack of suitable structure for pH and strong structure for As, Se, V, OM , clay content, and soil EC. Spherical model was the best model to describe spatial variability of the variables. Mean-square error (MSE) and correlation coefficient were used to determine validation of the variograms. The result showed the greatest accuracy of estimation was for soil EC. Distribution map for the heavy metals were prepared using the obtained information from elements by point kriging method and by using of Surfer software. Interpolation in blocks by dimensions of 1000*1000 m was made. The mine, effective factors in high concentration of As are black shall formation and erosion of igneous rock formation of Karkas mountains branches. Agricultural and industrial activities are probably effective factors causing increase the V concentration in the region. Metallic industry near Mobarakeh area is effective factor on high concentration of Se in soil of this area. Direction of dominant wind have affected in spread of Vanadium and Selenium