Metallurgicaloperations are one of the most important production processes that produces rawmetallic materials from natural resources, while they are often energy-intensive.Metallurgical processes in addition to making main products is significantlyassociated with the production of residues or wastes. The use of microorganismsis one of the new and effective methods for recycling valuable metals fromthese products, as secondary resources. The microorganisms used in this studywere Aspergillus niger , a fungus isolated from pistachio shells andgrape cubes. The performance of these microorganisms in the recovery of iron,aluminum, vanadium, scandium, titanium, strontium and rubidium from glaciersproduced in the Jajarm alumina factory was studied. To this end, a completefactorial design with 4 replication at the central point was implemented. Inthis design, the effects of fungus type, mechanical activation (at two levelsof zero and six hours), method of leaching (at two levels of one- and two-step)and solid percent (at two levels of one and three percent) on red-mudbioleaching were investigated. The generated biomass, sugar consumption andorganic acids released by both strains, as the main bioleaching agents, weremeasured during the experiments and their kinetic models were studied. Thehighest recovery (97.37%) was achieved in aluminum dissolution using grapestrain, 6 h of mechanical activation, 1% solid content, and two-step leaching.Also, maximum vanadium recovery was obtained as 93.66% applying grape strain, 3h activation, 2% solid, and one-step method. The best titanium recovery,76.86%, gained at pistachio strain, 6 h activation, 1% solid, and one-stepmethod. Moreover, the highest strontium (69.77%) was extracted after 6 h ofactivation, and using grape strain, 1% solid, and ne-step leaching method.Rubidium recovery, was optimized (62.04) at pistachio strain, 6 h activation,1% solid, and one-step method. Furthermore, the maximum scandium (51.75) wasrecovered after 6 h activation and using pistachio strain, 1% solid, andone-step leaching method. Finally, the best iron recovery was obtained as31.27% by implementing grape strain, 6 h activation, 1% solid, and two-stepleaching approach. Considering the dissolution of iron and aluminum metals, thetype of fungus, mechanical activation and solids content were the effectiveparameters. Also, regarding the dissolution of vanadium, fungus type, andmechanical activation,are the effective variables. Moreover, for scandium: typeof fungus and the method of bioleaching, for titanium, solid content, and forstrontium and rubidium, the type of fungi and solids had the greatest influence.Therefore, bioleaching using A. niger is a promising approach for the recoveryof many valuable elements from red mud residues, and after optimization anddevelopment, can be implemented as a low-cost and effective method in future.