Restoration of bone defects and bone remodeling is one of the most important challenges in the present era. Bone tissue engineering is one of the best choices for the reconstruction of bone tissue. Nowadays, the use of intelligent materials such as piezoelectric ones to accelerate the process of bone defect healing through prouducing electrical pulses in tissue engineering is increasing. The purpose of the present study was to produce and characterize a poly (vinylidine fluoride-poly-vinyl alcohol barium-calcium titanate fibrous scaffold. In this regard, the nanopowders of barium-calcium titanate containing 0%, 10%, 20%, 40% and 100% molar calcium were synthesized by sol-gel method.Through using X-ray diffraction analysis, scanning electron microscopy, Raman test and X-ray diffraction spectroscopy, calcium replacement was confirmed in the structure. Due to the presence of calcium in the structure at more than 10% concentratins secondary calcium titanate phase along with barium titanate with reduction of crystallite size and grain size were observed. The electrical behavior of the particles was also investigated through considering the dielectric constant of each samples. Also bioactivity of synthesized samples were evaluated by immersion in simulated body solution. In addition, the effect of the amount of calcium in the barium titanate structure, on the behavior of MG63 cells was investigated. The results of the MTT test showed that replacing calcium ions in the structure of barium titanate for more than 20% could increase the compatibility of particles in higher concentrations. Cell adhesion also showed more appropriate behavior of the 10% and 20% samples containing calcium in the structure. After the investigations, the optimum calcium percentage for barium-calcium titanate particles was selected according to cell and electrical behavior, so that in the fabrication of poly (vinylidine fluoride-poly-vinyl alcohol barium-calcium titanate scaffold, the selected barium-calcium titanate sample was used by electrospinning method. Also, the effect of adding different percentages of optimum nanoparticles on morphology of the scaffold, physical properties , growth and cell adhesion were evaluated. The lowest fiber diameter (560.26 ± 130 nm) and the highest hydrophilicity were observed in the sample containing 5 wt.% of nanoparticles. According to cytotoxicity assay cell proliferation significantly promoted by increasing the time of test. Due to agglomeration of nanoparticles at higher concentrations of barium-calcium titanate mechanical properties were decreased. The highest strength was observed in the scaffold containing 1 wt% of nanoparticle (1.8 ± 4.65 MPa) which was selected as the optimal percentage of barium-calcium titanate according to mechanical and cellular results. Accordingly, poly (vinylidine fluoride-poly-vinyl alcohol barium-calcium titanate fibrous scaffold-1 wt% barium-calcium titanate ( which includes 10% molar calcium in the structure) can be a suitable scaffold for repairing bone defects in bone tissue engineering Keywords : bone tissue engineering, sol-gel, barium-calcium titanate, poly vinylidine fluoride,electrospinning , poly-vinyl alcohol