The level of glutathione (GSH) is important in blood and urine, as biomarker in a number of clinical diagnoses. Isoperterenol (ISP) is a drug that plays major role in the treatments of bronchi constriction in patients with asthma and may be used in emergency patients with heart block. Therefore determination of GSH and ISP is important. In this work, CL method has been used for the determination of GSH and ISP. It is based on the enhanced CL of the reaction of luminol and diperiodatoargentate(III) (DPA) {K[Ag (H 3 IO 6 ) 2 ]} in an alkaline solution. At first, the effects of chemical and instrumental variables on the CL intensity were studied. In chapter 1, in GSH determination, the catalytic effect of inclusion complex of beta-cyclodexterin ( ? ?CD)/ MnFe 2 O 4 (M) magnetic nanoparticles in different medium on the CL response of luminol-DPA system was investigated. The MnFe 2 O 4 (MNP S ) were prepared at physics laboratory of Isfahan University of Technology. The ? ?CD formed an inclusion complex with MnFe 2 O 4 M, via absorption on the surface of MnFe 2 O 4 M, which could stabilize them. In recent years, there have been paid considerable attentions to the metallic and magnetic nanoparticles (M) because of their catalytic effect, super paramagnetic behavior, their large ratio of surface to volume, and low toxicity. Using this catalyst dramatically make the CL intensity increase (9 times) and response time (the time that peak was reached its maximum level) decrease about 10 times. Under the optimum conditions, dynamic ranges were obtained from the range of 5.0× 10 -8 to 4.0×10 -6 mol/L of GSH. The detection limit of the method is 1.5×10 -8 mol/L (3S b /m) and the relative standard deviation for 9 replicates measurement of 8.0×10 -7 mol/L GSH was 2.8%. The proposed method is compared with the Ellman reference method for the determination of GSH. It was successfully applied for the rapid and sensitive determination of GSH in human blood samples. In chapter 2, the linear correlation between the CL emission intensities and ISP concentrations was investigated. Under the optimum conditions, the CL intensity vs. ISP concentration was linear in the range of 1.0 × 10 -8 to 9.0 ×10 ?7 mol/L. The detection limit was 4.7×10 -9 mol/L for ISP and the relative standard deviation for n=11 replicates measurement of 2.0×10 -7 mol/L was 2.7%. The proposed system was successfully applied to determine of ISP in human serum and pharmaceutical. The reaction mechanism for these CL reactions are proposed and discussed.
