In this work, hot water extraction and dispersive liquid- liquid microextraction coupled with high performance liquid chromatography-diode array detector (HPLC-DAD) were applied for the extraction and determination of sulfonamides (sulfacetamide, sulfadiazine, sulfamethoxazole and sulfaquinoxaline) in chicken meat and water samples. For the microextraction, extraction solvent (chloroform) contains 5% surfactant (methyl trialkyle ammonium chloride) was injected in to a 10 mL glass tube with conical bottom containing aqueous solution of sulfonamides by a 50 ?L syringe. After centrifugation, the sedimented phase was complectely transferred to another tube with conical bottom using a 25 ?L HPLC syringe. 10 ?L mixture of methanol-water (30:70) was added in to the sedimented phase and finaly 20 ?L of the solution was injected into the HPLC. Extraction of these compounds from the meat was performed with hot water at 100 o C. In order to obtain optimum conditions of the microextraction, several parameters affecting on the microextraction such as: extraction solvent type, percentage of disperser solvent in extraction solvent, pH , sedimented phase volume, salt addition and time of the extraction were studied. Parameters affecting on the extraction of these compounds from the meat contaning temperature, time, pH and volume of water were studied. Under optimum conditions for microextraction (extracting solvent, chloroform? amount of surfactant in chloroform, 5% v/v? sedimented phase volume, 10 ?L? pH, 7? without salt addition) Enrichment factors and limit of detections (LODs) were ranged 27.9- 56.7 and 0.01-0.2 ?g/L, respectively. The relative standard deviations (R.S.D.s) for analysis of 20 ?g/L of sulfonamides in water samples were in the range of 3.6-5.3% (n=5). Optimum condition for extraction of these compounds from the meat were achieved as: 10 mL buffer (pH 4) at 100 o C for 10 minutes. The limit of detections (LODs) for chicken meat were in the range of 25.8-40.3 ?g/kg. As a result. the proposed method was used for analysis of rael samples such as zayandeh rood (Esfahan) river water, fish pool water , native chicken meat and chicken meat from Homa and Esfahan company.