Because of biocompatibility and desirable bioactivity, bioceramics are the most suitable materials for the replacement or repair of damaged hard tissue of the body, treatment of disease and disability and even replacement of the soft tissues of the human body. Also in recent years, because of biological properties, high bioactivity and osteoinduction properties, bioactive glass (bioglass) have been favored. On the other hand, the application of Hydroxyapatite (HA), mineral phase of the bone, has been expanding in types of natural and synthetic kinds. This study aimed at preparation, osteoinduction evaluation and antibacterial evaluation of nanobioglass and hydroxyapatite nanoparticles. 63S, 58S and 45S bioactive glasses and HA nanoparticles were obtained by sol-gel process. Bioceramic specimens were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), fourier transform infrared spectroscopy (FTIR) and laser doppler electrophoresis (LDE). As an index of bioactivity, HA formation ability on bioceramic specimens was evaluated in simulated body fluid (SBF). The capacity of prepared nanoparticles for bone formation was investigated in vitro using human cord blood derived unrestricted somatic stem cells (USSC) under osteogenic induction and toxicology of specimens was