A novel and simple electrochemical sensor is constructed for selective determination of furosemide by combination of a multi-wall carbon nanotube (MWCNT) layer with a thin molecularly imprinted polymer (MIP) film. The sensor is fabricated onto pencil graphite electrode (PGE) via stepwise modification of MWCNT and a thin polymer film of molecularly imprinted polymers by using electrodeposition method.The molecularly imprinted film displayed excellent selectivity towards furosemide. Due to such combination,the sensor responded quickly to furosemide.The morphology and performance of the sensor is characterized by cyclic voltammetry (CV),square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). The interaction between the imprinted sensor and target molecule is observed by measuring the variation of current response of the oxidation – reduction of the K 3 Fe(CN) 6 as a probe , on electrode.Under the optimal experimental conditions the response peak current is linear to the concentration of furosemide in the range from 0.08 to 4.00 mM and the detection limit is 35 nM.This imprinted sensor is successfully tested to the determination of furosemide in human urine samples .These results revealed that the imprinted sensor fulfilled the selectivity,sensitivity ,speed and simplicity requirements for furosemide detection and provided possibilities of clinical application in physiological fluids.