In this study, an injection port was designed and constructed for direct analysis of sorptive sample, by ion mobility spectrometer (IMS). The inner diameter of injection port for the entrance of sample in to the IMS is less than 1 mm, resulting in reduction the dead volume and peak broadening. Sol-gel procedure was applied to prepare the polydimethylsiloxane absorbent on the rotating rod. The figures of merit for direct analysis of diazinon (as a test compound) were obtained as the detection limit of 30 ?g L -1 and linear dynamic range of 100-1250 ?g L -1 with a correlation coefficient of 0.9975. The affects of some parameters on the extraction efficiency including stirring rate, extraction temperature, extraction time, salt addition and desorption temperature were studied and optimized. The detection limit of the method was calculated to be 1.5 ?g L -1 . Also, the linear dynamic range was obtained to be 5-200 ?g L -1 with a correlation coefficient of 0.9991. The intra- and inter-day relative standard deviations were calculated to be 4 and 5%, respectively. Agricultural wastewater, well water and apple were selected as real samples. The linear dynamic range were obtained in the range of 5-250 ?g L -1 , 5-200 ?g L -1 and 25-1000 ?g kg -1 for the agricultural wastewater, well water and apple samples, respectively. Also, the limit of detection was obtained to be 1.5 ?g L -1 for agricultural wastewater and well water, and 7.5 ?g kg -1 for apple sample. The recovery values were calculated 96 ± 6, 107 ± 3 and 105 ± 6 for the agricultural wastewater, well water and apple samples, respectively. These results reveal the capability of the constructed injection port for routine analysis of sorptive extraction samples.