One of the most important concern on medical engineering is due to infections caused by replacing implants into defects. Many researchers have been done some efforts to obtain appropriate and economical coating. The aim of this study was to prepare and characterize Chitosan/Copper nanocomposite coating by electrophoretic deposition. At the first, copper nanoparticle was synthesized in situ and none in situ made of CuSO 4 .5H 2 O as precursor. Secondly, aqueous and none aqueous suspensions were prepared for electrophoretic deposition. Stainless steel 316L was chosen as substrate and electrode. In order to investigation and characterization of copper nanoparticle and nanocomposite coating, X-ray diffraction, optical microscope, scanning electron microscope, field emission scanning electron microscope, transmission electron microscope, scratch test and antibacterial test were applied. The best results achieved from synthesis of copper nanoparticle was 11±6 nm which related to the solution with 4 g/l chitosan as stabilizer agent. Moreover, in the sample prepared with 1 g/l chitosan and 1% copper nanoparticle under 20 V and 5 min, resultant findings showed improvement of antibacterial activity against both gram-positive and gram-negative bacteria by increasing copper nanoparticle concentration, however, over 2% of copper nanoparticle, coating adhesion dramatically decreased. In conclusion, chitosan/copper nanocomposite have potential to be as coating for implants to prevent infections in orthopedic surgeries.