In this work, these compounds were determined in a multi-step procedure using solvent-extraction and then the analytes were clean-up with mixed mode cation exchange SCX+C 8 cartridge for enrofloxacin and Sep-Pak C 18 cartridge for furazolidone, chlortetracycline and chloramphenicol. The analytes were eluted into a test tube and evaporated to dryness at 60 o C under N 2 stream. Then, the residue was dissolved in 100 ?L of methanol and then 20 ?L of the solution was injected into the HPLC instrument.The column was ODS and the mobile phase was water/methanol in PH=3.0 adjusted with orto-phosphoric acid. The UV-VIS detector was used for chloramphenicol, chlortetracycline and furazolidon. Enrofloxacin was detected with fluorescence detector. Relative standard deviations for analysis of standard solution are less than 4.4% . This procedure was used for the blank, spiked and real samples. The limit of detection of the analyte was sufficient to respond below the tolerance level of the analytes in the meat chicken and it was about 0.4 , 0.2 , 1.9 and 43.8 ?g/kg for chloramphenicol, furazolidone, enrofloxacin and chlortetracycline respectively. Relative standard deviations for analysis of spiked samples, controlled samples and market samples are less than 12.6% , 8.8% and 12.2% respectively.