This study defined the design, development and Comparison of molecularly imprinted polymers of carbon dots (CDs) and polymer dots (PDs) for measure acetamiprid. PDs were prepared by a one-step aqueous synthetic way from ascorbic acid and diethylenetriamine at room temperature. That also CDs were prepared by hydrothermal method at 180 °C . in next step, a thin padding of silica was covered by reverse microemulsion method, in outer environment of PDs and CDs. At the end of this step, molecularly imprinted polymer inside PDs and CDs were obtained. Other variables affecting the fluorescence peaking were optimized by design expert software. The results illustrated that the considered method for PDs had wide dynamic range 0.08-101 nmol L ?1 , good accuracy and LOD 0.024 nmol L ?1 , also CDs had Thin dynamic range 0.12-2.19 nmol L ?1 and LOD 0.031 nmol L ?1 . The response of the optical sensor on usual specimens in water and Fruitage was considered. The consequence certified the well selectivity of the sensor for the evaluation of acetamiprid. In the second work, the simultaneous analysis of levodopa and pyridoxine drugs using molecularly imprinted polymers CDs was performed for the first time. The NCDs using citric acid and urea were prepared by hydrothermal method and also O-CDs were synthesized by o-phenylenediamines and Ethanol via hydrothermal method. NCDs@MIP and O-CDs@MIP were synthesized via microemulsion method on the outer environment of NCDs and O-CDs. Other variables affecting the fluorescence peaking were optimized by design expert software. The results illustrated that combine NCDs@MIP and O-CDs@MIP for simultaneous analysis of levodopa and pyridoxine had dynamic range 0.05-0.46 µmol L ?1 for pyridoxine and 0.04-0.37 µmol L ?1 for levodopa. Also this method had good accuracy and detection limit 0.02 µmol L ?1 for pyridoxine and 0.01µmol L ?1 for levodopa. The response of the optical sensor on common samples including blood plasma and Urine was considered. The results certified that this method have well selectivity of sensor for simultaneous analysis of levodopa and pyridoxine.
