In recent years, medical textiles have gained huge interest and different approaches have been applied for modification of properties of medical textiles. Chitosan is one of chitin derivatives and due to its desirable properties such as biocompatibility, biodegradability, availability , antibacterial properties and its hemostasis have been broadly applied in biomedical fields. Carboxy methyl chitosan(CMC) is one of the chitosan derivatives with attractive properties such as high absorbable of metal ions and more solubility than chitosan. In this research, poly vinyl alcohol (PVA) as a biocompatible, biodegradable and water soluble polymer was blended with CMC for improvement of spinnability of CMC soliution as spinning of pure CMC solution is difficult. Alo.vera as an anti- inflammatory agent and ciprofloxacin as an antibiotice was incorporated in the structure of PVA/CMC nanofibers through their blending with PVA/CMC and electrospinnig of resultant solution. After optimization of concentration of different solutions and electrospinning set-up PVA, PVA/CMC, PVA/CMC/Alo.vera and PVA/CMC/Alo.vera/ciprofloxacine nanofiber mats were fabricated. The morphology of different nanofiber mats was observed using SEM and fiber diameter was measured by Digimizer softwear. FTIR was used for identification of different component and confirmation of their presence in the structure of nanofibers. Antibacterial test was performed using Pseudomonas aeruginosa and Staphylococcus aureus bacteria to compare antibacterial properties of different nanofiber mat. Fiber diameter for PVA, PVA/CMC, PVA/CMC/Alovera and PVA/CMC/Alo.vera/ciprofloxacin was found to be 513.20(SD=120.534), 507.4(SD=63.265), 473.72(SD68.589) and 519.5(SD142.732) (nm) respectively. FTIR spectra confirmed the presence of individual component in the different nanofiber web. antibacterial properperties of CMC/PVA was found to be higher compare to that for PVA nanofiber mat revealing antibacterial effect of CMC. Addition of Alo.vera also increased antibacterial activity of nanofiber mat for Pseudomonas aeruginosa . However no anti bacterial effect for Alo.vera was found for Staphylococcus aureus indicating antibacterial effect of Alo.vera depends on the types of bacteria. Nanofiber mats containing ciprofloxacin showed the best antibacterial properties in comparision with PVA/CMC and PVA/CMC/Alo.vera.In conclusion, PVA/CMC/Alo.vera/Ciprofloxacin nanofiber mat was found as a appropriate substarte for antibacterial applications. Key words :Chitosan, carboxymethyl Chitosan, poly vinyl alchol, A.vera, Ciprofloxacin, Nanofiber, Antibacterial