Due to the high strength and relatively affordable price, concrete currently is the most applicable building materials, so that as more than 1 cubic meter concrete for every person in the world would produce annually per year. At least 7% of whole carbon dioxide spread in the globe is contributed to cement production used in the concrete. Hence in this way, the trend toward decreasing the cement consumption in the concrete and replacing it with a cement-like materials such as fly ash, blast furnace slag, micro silica, limestone, etc. has been increased. Slag is by-product of blast furnace and fine limestone is waste of stone cutting in Iran; nowadays use of these two cement replacement spread due to technical, economic and environmental advantages. To increase the durability of concrete and repair pores and micro-cracks microbiological method can be utilized. In this method, the bacteria by producing urease enzyme plays a role like catalyst and helps the urea hydrolysis. If this occurs hydrolysis of calcium-rich environment, solid calcium carbonate will form. The made Calcium carbonate deposits, fills pores and cracks of concrete. In order to achieve the objectives of the study, three groups of samples in general were planned and built. Bacteria in the first batch of samples to compare favorable, was not used. Concretes with six mixes contain two ratios of 0 and 15% limestone powder and three ratios of 0, 20 and 40% slag were made. In this category 216 cylindrical specimens with a diameter of 100 and 200 mm height, 36 mm cube of dimension 100, 36 cubes with dimensions of 70 mm, 36 cubes with dimensions of 70 mm contain steel and 36 cubes with dimensions of 120x200x200 mm were made. Environmental samples for 28 days in tap water, sea water and soluble calcium lactate and urea were treated. In the second sample, mixing in a concentration of bacteria was mixed with water and mix concretes six with the same percentages listed in the first batch were built of limestone powder and slag. Samples and processing environments in this category, like the first group. In the third instance, a mix containing 15% limestone and 20% slag were intended for surface treatment using bacteria. In this category 36 cylindrical specimens with a diameter of 100 and 200 mm height, 6 mm cube of dimension 100mm, 6 cubes with dimensions of 70 mm, and 6 cubes with dimensions of 70 mm contain steel and 6 cubes with dimensions of 120x200x200 mm were made. At the end of processing the surface of samples in the mentioned media for two weeks treats by bacteria. In order to evaluation improvement of concrete durability, water absorption, compressive strength, electrical resistance, the influence of chloride ion, sulphate compressive strength, determination of carbonation depth, the depth of water penetration and bar corrosion tests was performed on the samples. The results show that bacteria-mixed concrete and concrete surface treatment improves durability. Samples with bacteria than samples without bacteria, have less water absorption, carbonation depth, and the depth of penetration, and have more compressive strength, electrical resistance and charges of potential rebar. In relation to cement replacement materials it was concluded that, limestone act as a source of calcium for bacteria and slag enhance the compressive strength and reduce infiltration. Among the curing environment, calcium lactate and urea have better effect on samples containing bacteria and surface treatment. Key words concrete, bacteria, limestone, slag, surface treatment