Rose is one of the top five flowers in the world. This flower is especially popular among people all over the world due to its various shapes, smells and colors. In addition, cultivation conditions and production methods are very important in quality, post-harvest life and finally the price of the product. Therefore, this study was conducted to investigate the effect of different concentrations of calcium chloride on yield, quality and post-harvest life of Jumilia cut flowers in Sabzinehsazan Greenhouse located in Goldasht, Isfahan during 2019-2020. This factorial experiment was performed in a fully-randomized block design with 5 levels of calcium chloride (control: distilled water, 5, 10, 20 and 40 mM) and 2 spraying times of 10 and 10-20 days after bending in 4 replications. In this experiment, stem length and initial fresh weight of flowering stem, relative flower diameter, uptake solution, post-harvest life, relative water content, ion leakage, chlorophyll a, b and total chlorophyll, carotenoids, anthocyanins, calcium content, magnesium, Nitrogen, and leaf iron were measured. The results of the statistical analysis of the data showed that calcium chloride can increase 2 days post-harvest life of flowers compared to the control by increasing the strength of the cell wall and the middle blade as well as reducing ethylene production. In the initial fresh weight factors of the flowering stem, chlorophyll b and total chlorophyll and the amount of leaf iron when calcium spraying was done twice, had significant effects on the flowers. Also, application of 10 and 20 mM concentrations of calcium chloride increased the amount of chlorophyll a, nitrogen and leaf iron compared to other treatments. In ion leakage factors and vase life solution changes, 10 mM treatment had significant effects compared to other treatments. Increasing the concentration of calcium chloride to 40 mM could affect the relative diameter and amount of calcium in the leaf tissue. Also, the use of calcium chloride treatment had a significant effect on the relative leaf content. In addition, when 20 mM calcium chloride treatment was used, it showed the highest amount of carotenoids compared to other treatments. Key words Rose, Jumilia, Calcium Chloride, Post-harvest Life 66