The aim of this study was to in vitro evaluation the effect of pomegranate seed peel () on blood metabolites, sperm quality and sperm fertility in male goats. Nine cloned male goats (2 - 3 years old) were randomly allocated to three experimental groups: 1- 0% (control), 2- 30 (30) and 3- 60% (60). The barley grain was replaced with (0, 30 and 60%) in the experimental diets. However, all diets were adjusted to be iso-caloric and iso-nitrogenous using SRNS software. Semen samples were collected by artificial vagina twice a week. Semen quality including sperm motility, sperm membrane integrity, sperm viability, ROS production level and fertilization possibility rates were evaluated. Results showed that feeding 30 and 60% of could improve (P 0.05) some parameters such as sperm motility, sperm viability and the integrity of plasma membrane compared to the control group. The ROS production levels decreased (P 0.05) in the 30 and 60 groups compared to the control group. The in vitro sperm fertilization had no positive effect in all experimental groups and there was no result for fertilization, embryo development, blastocysts formation, and embryo hatching. The results of blood metabolites showed that 30 group had lower (P 0.05) blood urea nitrogen (16.81 mg/dl) than those the control (19.66 mg/dl) and 60 (20.27 mg/dl) groups. The group 30 had higher (P 0.05) serum albumin compared to the other groups. The 30 and 60 groups (63.28 60.85 mg/dl) had higher serum concentration of cholesterol than that the control (46.11 mg/dl). However, the concentrations of serum testosterone was not affected by the treatment (P 0.05). Moreover, serum malondialdehyde concentration was not affected by the treatment. The results suggest that feeding pomegranate seed peel as a source of unsaturated fatty acids and with antioxidant activity could improve some sperm characteristics and blood parameters in goat. Key words: Pomegranate seed peel, Antioxidant, Sperm, Blood metabolites, Cloned male goats.
