Drought has been recognized as a primary constraint in limiting the growth and development of plants. It impairs normal growth, disturbs water relations, and reduces water use efficiency in plants. Drought stress at any growth stage also, poses detrimental effects on morphological, physiological, and biochemical processes in plants. To maintain growth and productivity, plants must adapt to stress conditions and exercise specific tolerance mechanisms of stress agents. Plant modification for enhanced tolerance is mostly based on gene transformation, however, the nature of the genetically complex mechanisms of abiotic stress tolerance, and the potential detrimental side effects, make this task extremely difficult. A promising alternative for improving plant drought tolerance is using the beneficial soil microorganisms including Plant-growth-promoting fungi (PGPF). This research was undertaken to investigate the effect of two PGPFs on some physiological characteristics, nutritional status of sesame plant and also, activity of some soil enzymes under drought conditions. For this purpose, a field experiment using a completely randomized blocks with three replications and treatments including fungal inoculation (non-inoculation, Piriformospora indica and Glomus intraradices ) and drought levels include 55, 75 and 85% of MAD (Maximum available water depletion) on the basis of combined analysis in Lavark Field (Isfahan Province) was conducted. The results showed that drought stress resulted in greater reduction of shoot biomass, plant height, number of leaves, catalase activity, relative water content, membrane stability index, and concentration of nitrogen and iron in shoot part of non-inoculated plants compared to inoculated ones. The comparison of studied fungi indicates that only Glomus intraradicens increased the concentration of phosphorus in the shoot part of plants, significantly. Each of the studied fungi showed their positive effect on different characteristics and also different drought stresses. So that, Glomus intraradicens increased phosphorus content at 75% MAD, relative water content of leaf and nitrogen content at 85% MAD and catalase activity, membrane stability, iron concentration, shoot biomass and number of leaves at 75% and 85% MAD. However, P. indica increased iron concentration and height of shoot at 75% MAD, shoot biomass and nitrogen content at 85% MAD and catalase activity, membrane stability, relative water content of leaf, number of leaves and urease enzyme activity at 75% and 85% MAD. The studied fungi increased the activity of catalase and urease enzymes in all drought treatments. However, L-asparaginase activity was not changed in response to experimental treatments (drought and fungal inoculation). In general, the obtained results of this research indicate that drought is a limiting factor for plants, and PGPFs can improve plant tolerance to this environmental stress by improving plant nutritional status, water content and activity of anti-oxidant enzymes . Keywords: Drought, Plant Growth promoting fungi (PGPFs), Sesame and Soil enzymes