Zinc deficiency is considered one of the most widespread problems of human malnutrition worldwide. Zinc deficiency in humans results from diets that are low in bioavailable zinc. Direct relationship between zinc deficiency in humans and low zinc levels in soils and food crops has been recognised recently. Therefore one of the most important approaches for combating zinc deficiency is supplying Zn for crops via correcting Zn deficiency in soils. But for increasing plant uptake of Zn, the bioavailability of Zn in soil has to be increased. Deficiency of Zn rarely results from a limitation in the total amount of soil zinc, but it is almost always due to a shortage in readily plant-available Zn. Root exudates and organic acids released during decomposition of soil organic matter can affect the availability of zinc for plant uptake. Although main form of zinc taken up by roots is divalent ion, it is believed that zinc-chelate complexes may also be taken up by plants. Considering this suggestion, the effect of addition of organic ligands into the soil on the bioavailability of Zn requires knowledge on the function of these ligands and the mechanism of their uptake by plant roots. Thus, this nutrient solution culture study was conducted to investigate the effect of some natural and synthetic chelates on zinc uptake and translocation in two wheat genotypes with different zinc-efficiency. Two wheat genotypes ( Triticum aestivum L. Back Cross Rushan as zinc-efficient and Kavir as zinc-inefficient) were exposed to two levels of zinc (10 and 100 µM in the form of zinc sulphate) and six different carboxylic acids (citric, tartaric, oxalic, salicylic, ethylenediamin tetraacetic acid and L-methionine) in a nutrient solution. The experiment was set up in a completely randomized factorial design; each treatment contained three replicates. Analysis of variance procedures were conducted with the PROC GLM procedure of SAS. Mean separations were performed using Fisher’s protected least significant difference (LSD) method at P 0.05. Results of this study showed that on the one hand, the Kavir genotype had higher shoot and root dry matter, shoot zinc uptake, root Fe concentration and uptake, shoot and root Cu uptake, and root Mn uptake in comparison with the Back Cross Rushan genotype whereas the shoot Zn, Fe, and Mn concentrations were the lowest in the Kavir. Back Cross Rushan genotype had the highest shoot Zn, Fe, and Mn concentrations. On the other hand, tartaric acid and citric acid caused the highest root Zn concentration and uptake in the Back Cross Rushan genotype, while the lowest in the Kavir genotype. The L-methinine and oxalic acid also caused the highest shoot Cu and Fe concentrations in the Kavir genotype in comparison with other wheat