Endophytic micro-organisms spend, at least a part of their life, inside the plant, without showing any signs. Presence of these symbionts in the host plant could be a vital factor, impacting on the plant-insect or in general, plant-arthropod interactions. Endophyte fungus of the geanus Epichole are more important, and produce a huge volume of toxic alkaloids. These alkaloids have toxic, repellent and deterrent effects on invertebrates. In this research, after confirmation of the tall fescues in the first test, and perennial ryegrasses for two spotted spider mite and onion trips, the direct effect of the endophyte infected plants and endophyte free ones on these pests were investigated. Among six treatments the mite feeding on the tall fescue leaves, 75C genotype resulted the highest mortality percent of 78%. In the choice test, using the leaves of the endophyte infected plants and endophyte free plants, the lowest percent of presence after 8 hours, belonged to the endophyte infected 75C genotype. In the second test, the effect of application of the residues and extracts of endophytes infected and endophyte free tall fescues and perennial ryegrasses on the eggplant, cucumber, and tomato were studied. In addition, their repellent and killing effect on different growing stages of three pests of two spotted spider mite, tomato leaf miner and onion trips were studied. The plant residues of 10 and 20 grams per 500 grams were added to the planting bed. Among the residue treatments, the highest lethal activity was resulted by 20-grams summer residue of the endophyte infected tall fescue, 75C genotype, equal to 68.8 percent. In the choice test for the two spotted spider mite on the cucumber leaves, treated by the plant residue, and the bean leaves, treated by the plant extracts, the mites preferred the plants treated by the endophyte free plant residue or plant extracts. The lowest percentage of mite presence was observed in the tall fescue endophyte infected 75C residue treatment (16.4%), and about the extracts, tall fescue endophyte free 75B genotype extract (18.1%). The highest mortality of summer residues of tall fescue endophyte infected 75B genotype, was seen (31.11%) on T. tabaci . The highest mortality of T. absoluta larvae (60.0%) was achieved on the tomato plants treated with the summer residue of 75C genotype. With increasing the amount of added residue to the soil or extract concentration, the mortality percent of three tested pests were increased. Adding endophyte infected plant residues to the soil had significant effects on morphological characteristics of eggplant, cucumber, and tomato. According to the achieved results, application of the residues of the endophyte infected plants to the planting bed of the greenhouse and farm plants or using their extracts as the insecticide or acaricide can produce negative effects on the tomato leaf miner, two spotted spider mite and onion trips. It is necessary to commit more research on the symbiosis of the endophyte fungi and utilizing their residues or extracts in the pest management. Keywords: Tall fescue, Perennial ryegrass, Two-spotted spider mite, Tomato leaf miner, Onion thrips, Planting bed