Phosphorus (P) deficiency is one of important abiotic stresses resulted in crop yield reduction in most agricultural lands, worldwide. Therefore, finding effective approaches to increase plant tolerance to P deficiency is of great importance. Mycorrhizal fungi inoculation of plant roots can be applied as an effective strategy for reducing detrimental effects of phosphorus deficiency. This study was conducted in greenhouse of Soilless Culture Research Center, Isfahan University of Technology to investigate the influence of individual and combined inoculation of endophytic fungus, Piriformospora indica and mycorrhizal fungi ( Glomus mossea and Glomus intraradices ) on some morphological and physiological properties of two wheat cultivars ( Triticum aestivum cv. Azadi and Nicknejad) in response to phosphorus deficiency stress. Wheat plants were grown in pots containing a mixture of sterile sand: perlite (2:1 v/v) and exposed to two levels of P (64 and 32 mg/l). The results showed that shoot iron (Fe) and P concentrations, the rate of leaf photosynthesis, and root and shoot dry mass of both wheat cultivars decreased under P deficiency conditions. In contrast, P deficiency increased shoot zinc (Zn) concentration, total chlorophyll content, and activity of catalase (CAT) and ascorbate peroxidase (APX). No significant differences were found between two wheat cultivars in terms of shoot dry mass, P and Zn uptake, activity of CAT and APX and stress tolerance index under P deficiency conditions. Inoculation of plants root with mycorrhizal fungi and P. indica resulted in higher crop tolerance to P deficiency by increasing activity of antioxidant enzymes, stimulating the photosynthesis rate and enhancing P uptake by the root. The response of the wheat cultivars to mycorrhizal inoculation was different and the positive effect of the studied fungi inoculations on growth and tolerance to P-deficiency of Azadi cultivar was greater than Nicknejad cultivar. The most effective fungal treatments for improvement of P concentration of wheat cultivars were individual inoculation of P. indica and simultaneous inoculation of P. indica and G. Mossea in Azadi and Nicknejad cultivars, respectively. Based on the obtained results, the most effective fungal treatment for increasing the shoot and root dry mass of the studied wheat cultivars under P deficiency were simultaneous inoculation of P. indica and G. intraradices . The most effective treatment to increase antioxidant enzymes activities of Azadi cultivar was individual inoculation of G. intraradices . The best microbial treatments in increasing enzyme activity of CAT, APX and guaiacol peroxidase (GPX) of Nicknejad cultivar was belonged to simultaneous inoculation of P. indica and G. mossaea , individual inoculation of G. mossea and co-inoculation of P. indica and G. intraradices . The results also showed that the studied fungi can improve wheat tolerance to P deficiency stress by enhancing nutrient uptake by the root, improving root and shoot plant growth, increasing activity of antioxidant enzymes and stimulating the photosynthesis rate. It should be mentioned that the effect of the studied fungi on wheat cultivars and their antioxidant response to P deficiency was different. Although, fungi treatments can be used to increase wheat tolerance to P deficiency, but different responses of the cultivars to fungi treatments should be investigated. Keywords : Phosphorus deficiency, Mycorrhizal fungi, Antioxidant enzymes, Piriformospora indica.