The objective of this study was to assess the effects of different recycled fat sources, dietary vitamin E and vitamin C supplementation on performance, egg quality, egg fatty acid profiles, and egg oxidative stability and blood parameters in laying hens. Two experiments (Exp.) were conducted to determine the AME content of refined soybean oil (SBO), recycled soybean oil (RSO), and acidulated soybean oil soapstocks (ASO), and the effects of inclusion of vitamin E and vitamin C in diets containing 3.5% of these oils on performance and egg quality of Single Comb White Leghorn hens from 44 to 56 wks of age. In Exp. 1, the AME of the 3 oils was estimated in adult cockerels (n=6) either by multiplying the digestibility of the oil by its gross energy value or by difference between the AME values of the basal diet and of a diet that included 5% of the oil. The estimated AME of the SBO, RSO, and ASO oils were 8,916, 8,880, and 7,849 kcal/kg when measured directly from oil digestibility coefficients and 9,127, 8,947, and 7,967 kcal/kg when calculated by difference, respectively. In Exp. 2, there were 12 dietary treatments arranged factorially with the 3 oil sources, 2 levels of supplemental vitamin E (10 vs. 250 mg/kg), and 2 levels of supplemental vitamin C (0 vs. 250 mg/kg). Each diet was replicated 5 times and the experimental unit was a cage with 5 hens. Diet did not affect laying hen performance or egg quality traits in any of the periods considered. The inclusion of fat sources to the diets did not have clear effects on the yolk fatty acid composition, except for linolenic acid that was higher for SBO. Supplementation of the diet with vitamin E caused an increase in saturated fatty acids (SFA) especially C16:0 and C18:0 but did not affect monounsaturated fatty acids (MUFA) except C16:1 that was increased (P 0.05). Vitamin E supplementation decreased the polyunsaturated fatty acids (PUFA) content, especially C18:2 and C22:6n3 (P 0.05). The ratio of PUFA: SFA decreased whereas the ratio of arachidonic:linoleic (AA:LA) increased with vitamin E supplementation. Vitamin C supplementation caused an increase in C18:0, C18:3n3, AA:LA ratio and total n3 FAs but a decrease in n6:n3 ratio. Serum parameters including cholesterol, triglyceride and SGOT, SGPT and alkaline phosphatase enzymes were not affected by treatment (P 0.05). Serum Malondialdehyde (MDA) content of RSO was higher than ASO and it was decreased by vitamin E and vitamin supplementation (P 0.05). No differences were observed on yolk and egg parameters including cholesterol, triglyceride and MDA by treatment (P 0.05). MDA content of yolk and egg increased after 30 days storing in cold room whereas dietary vitamin E supplementation increased yolk and egg oxidation stability. ?-tocopherol concentrations of serum, yolk and egg were higher by vitamin E supplementation. Sensorial quality of eggs was not affected by dietary fat sources. It might be concluded that when recycled soy oil and acidulated soy oil soapstocks conveniently processed, are good alternatives for refined soybean oil in diets for laying hens. Under the conditions of this research, the supplementation of the diet with extra amounts of vitamin E and C despite the changes of yolk fatty acid profile did not improve egg production or egg quality. In addition, the increasing of egg yolk alpha tocopherol is possible by dietary vitamin E supplementation. Key words: apparent metabolizable energy, laying hen performance, soy oil sources, vitamin C, vitamin E